菟丝子提取物清除自由基作用的研究  

Free radical scavenging of the extract from Cuscuta chinensis Lam

作　　者：张培全[1] 谭茵[1] 张超[1] 

机构地区：[1]广州医学院药学实验中心,广州510182

出　　处：《中南药学》2012年第3期171-174,共4页Central South Pharmacy

基　　金：广东省科技计划项目(编号:2009B060700078);广州医学院青年基金项目(编号:2010L95115)

摘　　要：目的研究菟丝子提取物的体外抗氧化活性。方法采用热水提取、酶-Sevage法除蛋白、不同比例乙醇分级沉淀等方法获得菟丝子多糖提取物;经95%乙醇回流提取,2乙酸乙酯、正丁醇萃取,得到乙酸乙酯和正丁醇提取物。利用1,1-二苯基-2-苦苯肼自由基(DPPH.)和超氧阴离子自由基(O2-.)分别测定了各组分的抗氧化活性。结果菟丝子提取物均具有一定的抗氧化活性,且呈显著的量效关系。菟丝子乙酸乙酯、正丁醇提取物清除自由基的能力大于氧化型谷胱甘肽,小于还原型谷胱甘肽;菟丝子多糖清除自由基的能力均小于氧化型谷胱甘肽,其中90%醇沉多糖清除自由基的能力较强。结论菟丝子乙酸乙酯、正丁醇提取物以及90%醇沉多糖是天然抗氧化剂的良好来源,可以进一步分离纯化。Objective To investigate the in vitro antioxidant activity of the extract from Cuscuta chinensis Lam.Methods The polysaccharides were obtained by distilling with hot water,removing proteins with enzyme-Sevage method,further precipitating with different ethanol ratios.The extracts of ethyl acetate and n-butyl alcohol were obtained by distilling with 95% ethanol and extracting with ethyl acetate and n-butyl alcohol.Meanwhile their antioxidant activity was measured by DPPH· and（O2-） methods.Results The extract from Cuscuta chinensis Lam had obvious antioxidant activity and showed significant dose-effect relationship.The antioxidant activity of ethyl acetate and n-butyl alcohol extract was better than that of GSSG,but worse than that of GSH.The antioxidant activity of polysaccharide was worse than GSSG.The polysaccharide precipitated by 90% ethanol had stronger antioxidant activity.Conclusion The polysaccharide precipitated by 90% ethanol and the ethyl acetate and n-butyl alcohol extract are good natural antioxidants,which is worth further separation and purification.

关 键 词：菟丝子 抗氧化活性 自由基 超氧阴离子自由基 DPPH 

分 类 号：R965.1[医药卫生—药理学]