尿脱落细胞端粒酶活性表达对尿路上皮癌的诊断价值  

Telomerase activity in urine exfoliurothelial cancer

作　　者：吴逵[1] 祝怀平[2] 诸禹平[1] 凌斌[2] 舒启安[1] 戴海明[2] 张祖明[1] 朱明[1] 吴竞生 沈法林[1] 

机构地区：[1]安徽省立医院泌尿外科,合肥230001 [2]安徽省立医院中心实验室

出　　处：《临床泌尿外科杂志》2000年第5期216-217,共2页Journal of Clinical Urology

摘　　要：目的 :探讨尿脱落细胞端粒酶活性表达对尿路上皮癌的诊断价值。方法 ;采用 PCR- EL ISA法检测尿液中脱落的肿瘤细胞端粒酶活性 ,并以肾癌及非肿瘤患者的尿标本作对照。结果 :5 9例尿路上皮癌患者尿脱落细胞端粒酶活性表达率为 88.1% ,而肾癌患者为 10 .0 % ,非肿瘤患者为 2 0 .0 % ,前者与后两者比较 ,有极显著性差异(P <0 .0 1) ;不同分化程度及不同临床分期膀胱癌患者尿脱落细胞端粒酶活性表达率无显著性差异 (P >0 .0 5 )。结论 :检测尿脱落细胞端粒酶活性表达可作为尿路上皮癌早期诊断。Purpose: In order to find a more sensitive and noninvasive assay for the diagnosis of urothelia cancer. Methods: It was analyzed by Polymerase chain reaction Enzyme linked immunosorbent assay (PCR ELISA) that telomerase activity in urine neoplasitic patients. Results: The positive rate of telomerase in urine exfoliated cells between urothelial cancer and renal cancer or nonneoplasia diseases existed significant difference (P< 0.01 ).There was no correlation between the expression of telomerase activity and differentiation of the tumor or clinical stage (P> 0.05 ). Conclusions: PCR ELISA assay to detect telomerase positive cells in urine exfoliated cells might be a useful tool in identification and monitoring of patients with urothelial neoplasm.

关 键 词：端粒酶 尿脱落细胞 尿路上皮癌 诊断 

分 类 号：R737.104[医药卫生—肿瘤]