非离子表面活性剂萃取-高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚  

Simultaneous Determination of Chlorogenic Acid,Quercetin and Kaempferol in Loquat Leaves by Non-ionic Surfactant Extraction Solvent Followed by High Performance Liquid Chromatography

作　　者：张丽贤[1] 周光明[1] 姜兰芳[1] 许丽[1] 

机构地区：[1]发光与实时分析教育部重点实验室,西南大学化学化工学院,重庆400715

出　　处：《食品科学》2012年第6期150-153,共4页Food Science

基　　金：国家科技重大专项(2008ZX07315);重庆市自然科学基金项目(CSTC,2007BB5370)

摘　　要：目的:以5g/100mL非离子表面活性剂Triton X-100为萃取剂,建立一种简单快速的采用超声提取,高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚的方法。方法:采用Phenomenex C18色谱柱(250mm×4.6mm,5.0μm),以甲醇-0.25%乙酸溶液为流动相梯度洗脱,流速1.0mL/min,紫外检测波长350nm。结果:绿原酸、槲皮素和山奈酚分别在0.011～220μg/mL(r=0.9997)、0.065～130μg/mL(r=0.9999)、0.068～136μg/mL(r=0.9999)线性关系良好,检出限(RSN=3)依次是1.94、0.22、0.27ng/mL;样品的加标回收率为90.40%～96.82%。结论:该方法具有样品前处理简单、绿色环保、灵敏快速等优点,可为枇杷叶中一些黄酮类物质和绿原酸的检测提供一个有效的方法。Objective：An HPLC method for the simultaneous determination of chlorogenic acid,quercetin and kaempferol in loquat leaves was proposed based on rapid ultrasonic-assisted extraction using the non-ionic surfactant Triton X-100 as the extractant.Methods：The chromatographic separation was achieved on a Phenomenex C18 column using a mobile phase made up of methanol and 0.25% acetic acid at a flow rate of 1.0 mL/min,and the analytes were detected with a UV detector at 350 nm.Results：The calibration curves for chlorogenic acid,quercetin and kaempferol were linear in the range of 0.011-220μg/mL（r=0.9997）,0.065-130μg/mL（r=0.9999）,0.068-136μg/mL（r=0.9999）,respectively.The limit of detection（LOD） was 1.94,0.22,0.27 ng/mL（RSN=3）,respectively.The average recoveries across three spike levels ranged from 90.71% to 94.88%（n=3）.Conclusion：The method is simple,rapid,convenient,sensitive,and environment-friendly,and thus can provide a new and scientific means for assaying the contents of flavonoids and chlorogenic acid in loquat leaves.

关 键 词：枇杷叶 TRITON X-100表面活性剂 高效液相色谱(HPLC) 绿原酸 槲皮素 山奈酚 

分 类 号：R284.1[医药卫生—中药学]