不同鼻咽癌细胞株对鸡胚尿囊膜模型血管生成影响的比较研究  

Angiogenic effect of different nasopharyngeal carcinoma cell lines on the chick embryo chorioallantoic membrane

作　　者：林少雄[1] 李湘平[1] 鲁娟[1] 梁碧君[1] 刘雄[1] 

机构地区：[1]南方医科大学南方医院耳鼻咽喉科,广东广州510515

出　　处：《热带医学杂志》2012年第3期261-263,F0003,共4页Journal of Tropical Medicine

基　　金：广东省自然科学基金(9151051501000093)

摘　　要：目的比较不同鼻咽癌细胞株在鸡胚尿囊膜模型上促进血管生成能力的差异。方法选用6日龄鸡胚,开窗后分别种植5-8F、6-10B及CNE-2三种鼻咽癌细胞株,三种细胞株细胞数为2×106/鸡胚者各一组,细胞数为5×105/鸡胚者各一组,另一组为对照组(PBS),每组10个鸡胚。孵育6日后,统计分析新生血管数及血管面积/鸡胚面积比。结果种植细胞数为5×105/鸡胚时,5-8F、6-10B细胞部分鸡胚有移植瘤形成,CNE-2细胞鸡胚均未见成瘤。种植细胞数为2×106/鸡胚时,三种鼻咽癌细胞株均可100%成瘤,其新生血管数依次递减,分别为(38.7±2.50)、(33.5±4.43)、(29.7±2.71),差异有统计学意义(P<0.05);血管面积/鸡胚面积比分别为(22.2±2.18)%、(18.7±2.45)%、(16.9±2.62)%,均高于对照组的(9.5±1.86)%,差异有统计学意义(P<0.05),且5-8F面积比高于其他两种细胞株(P<0.05)。结论鼻咽癌5-8F、6-10B及CNE-2三种细胞株在鸡胚尿囊膜模型上血管生成能力依次减弱,实验研究可根据实际情况选用。Objective The purpose of this study was to compare the angiogenic effect of different nasopharyngeal carcinoma （NPC） cell lines on the chick embryo chorioallantoic membrane （CAM）. Methods Six days old fertilized eggs were used in this study. A square window on the shell was cut, and the the NPC cell lines 5-8F, 6-10B and CNE-2 were inocluated into the CAM （n=10） with PBS as blank control. The eggs were then incubated for 6 days before the enumeration of the vessels number（VN）. The ratios between the area covered by the vessel and the CAM （VA/CAM） were analyzed. Results All the 3 NPC cell lines grow on the CAMs （100%） with a cell number of 2×106/CAM. The VN was significantly decreased （P0.05）. The VA/CAM of 5-8F was significantly higher than the other two cell lines（P0.05）. The VA/CAM from the three NPC cell lines inoculated embryos were significantly higher than the control （P0.05）. Conclusion The results indicated that the angiogenic effects of these NPC cell line are different with each other, therefore the selection of these NPC cell lines may depend on the different CAM experimental conditions.

关 键 词：鸡胚尿囊膜模型 鼻咽癌 血管生成 

分 类 号：R739.63[医药卫生—肿瘤]