大肠癌中E2F-1、β-catenin和cyclinD1的表达关系  

Expression of E2F-1,β-catenin and cyclinD1 in colorectal adenocarcinoma

作　　者：韩慧芳[1] 刘岩[1] 张志叶 

机构地区：[1]河北省邯郸市第二医院,河北邯郸056001

出　　处：《现代中西医结合杂志》2012年第12期1261-1263,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省邯郸市科学技术局资助项目(1023108098-6)

摘　　要：目的探讨大肠癌组织中β-catenin异常表达的意义及其与E2F-1、cyclinD1表达的关系。方法采用免疫组化SP法检测78例大肠癌、20例正常大肠黏膜、30例大肠腺瘤中E2F-1、β-catenin和cyclinD1的表达情况。结果 20例正常大肠组织中β-catenin蛋白呈正常表达,而E2F-1、cyclinD1表达呈阴性。大肠腺瘤和大肠癌组织中E2F-1蛋白阳性表达为6.67%和61.54%;β-catenin蛋白异常表达为36.67%和92.31%;cyclinD1蛋白阳性表达为13.33%和55.13%。E2F-1、cyclinD1蛋白阳性表达和β-catenin蛋白异常表达均与大肠癌病理分级、临床病理分期以及淋巴结转移有关(P均<0.05)。大肠癌中β-cate-nin与cyclinD1呈正相关(P<0.05);β-catenin与E2F-1没有明显相关性(P>0.05),但E2F-1和cyclinD1呈明显相关性(P<0.05)。结论 E2F-1、β-catenin和cyclinD1的表达与大肠癌的发生、发展及浸润转移有关,可作为判断大肠癌生物学行为和预后的重要指标。Objective It is to approach the significance of abnormal expression of β-catenin in colorectal cancer tissue,and the relationship between the expression of cyclinD1,E2F-1 and β-catenin.Methods The immunohistochemical SP method were used to detect the expressions of E2F-1,β-catenin and cyclinD1 in 78 cases of colorectal adenocarcinoma,20 cases of normal colorectal mucosa and 30 cases of colorectal adenoma.Results In the 20 cases of normal colorectal tissues,the expression of β-catenin protein was normal,while the expression of E2F-1 and cyclinD1 was negative.In colorectal adenoma and colorectal cancer the positive expression of E2F-1 protein was 6.67% and 61.54%,the abnormal expression of β-catenin protein was 36.67% and 92.31%,the expression of cyclinD1 protein was 13.33% and 55.13%.The abnormal expression of β-catenin protein and the positive expression of E2F-1,cyclinD1 protein were related with the pathological grade of the colorectal cancer,clinical pathological staging and lymph node metastasis（P0.05）.In colorectal carcinoma,β-catenin and cyclinD1 were positively correlated（P0.05）;there was no significant correlation between β-catenin and E2F-1（P0.05）,but E2F-1 and cyclinD1 were significantly correlated（P0.05）.Conclusion The expression of E2F-1,β-catenin and cyclinD1 is related with the occurrence,development and metastasis of colorectal cancer and it can be the prognosis of the biological behavior of colorectal cancer.

关 键 词：大肠癌 E2F-1 Β-CATENIN CYCLIND1 免疫组化 

分 类 号：R735.34[医药卫生—肿瘤]