超滤法分离花生肽及其抗氧化活性的研究  

Antioxidant Activities of Peanut Protein Separated by Ultrafiltration

作　　者：张会翠[1] 唐琳[1] 杨庆利[2] 于丽娜[2] 孙杰[2] 刘少芳[2] 

机构地区：[1]山东师范大学,山东济南250014 [2]山东省花生研究所,山东青岛266100

出　　处：《花生学报》2012年第1期1-5,共5页Journal of Peanut Science

基　　金：青岛市公共领域科技支撑计划项目(09-1-1-84-nsh)

摘　　要：用碱性蛋白酶解法制备花生多肽,将得到的花生肽酶解液通过超滤的方法进行分离得到不同分子量段的多肽,对其进行抗氧化活性的研究得到抗氧化活性最强的分子量段的多肽。将花生短肽酶解液稀释并分别通过5KD、3KD的卷式纤维膜进行超滤得到三个分子量段的花生短肽。比较各分子量段的多肽对二苯代苦味酰基(DPPH)自由基的清除率、抗亚油酸氧化能力、羟自由基的清除率及还原力。结果表明:花生肽具有一定的抗氧化能力,且3KD以下的多肽抗氧化能力最强,3～5KD分子量段的多肽次之,大于5KD分子量的多肽表现出较弱的抗氧化能力。The peanut peptide was obtained with hydrolysis defatted peanut flakes, which was separated by different ultrafiltration membranes to three different size peptide. And then the reducing power, hydroxyl radical scavenging, antioxidant and DPPH radical scavenging were determined with different concentration and molecular weight of peanut peptide. The experimental results showed that the peanut peptide had relatively strong antioxidant activity, and with the different molecular weight of peanut peptide, they showed different antioxidant activity. The molecular weight less than 3KD showed the strongest antioxidant activity, 3-5KD was the next, and the molecular weight more than 5KD showed the weakest antioxidant activity.

关 键 词：花生肽 超滤 抗氧化 

分 类 号：S565.2[农业科学—作物学] Q516[生物学—生物化学]