呋喃C-30对变异链球菌生物膜早期形成的影响  

Effect of furanone C-30 on initial biofilm formation of Streptococcus mutans

作　　者：王玉霞[1] 王倩[1] 胡曰健[1] 马瑞[1] 唐子圣[1] 朱彩莲[1] 何智妍[1] 黄正蔚[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔医学院牙体牙髓病科上海市口腔医学重点实验室,上海200011

出　　处：《口腔生物医学》2012年第1期1-4,共4页Oral Biomedicine

基　　金：国家自然科学基金(81070826/30872886);上海市科学技术委员会资助(08DZ2271100);上海市重点学科建设项目(S30206)

摘　　要：目的:探讨密度感应拮抗剂呋喃C-30对变异链球菌生物膜早期形成的影响。方法:将体外合成的密度感应拮抗剂呋喃C-30按终浓度10、100μmol/L分别配制于含变异链球菌的牛心脑浸液培养基,37℃微需氧培养24h,形成生物膜后,用生物膜定量分析仪检测生物膜形成的量。结果:100μmol/L呋喃C-30组变异链球菌生物膜的形成受到显著抑制,磁珠成像开始减弱和完全消失的时间均迟于对照组;生物膜开始形成后,其生物膜形成指数低于对照组,差异有统计学意义(P<0.05)。结论:呋喃C-30在100μmol/L浓度时能有效抑制变异链球菌生物膜的形成,其应用可能为龋病防治提供新的思路。Objective：To research the effect of quorum sensing inhibitor furanone C-30 on initial biofilm formation of Streptococcus mutans.Methods：The in vitro synthetic quorum sensing inhibitor furanone C-30 was prepared with BHI medium in which Streptococcus mutans was cultivated into different final concentration of 10μmol/L,100μmol/L.Biofilms were formed after being incubaed at 37℃ for 24 h,followed which the kinetic quantification of biofilm formation was detected by BioFilm Ring Test.Results：The furanone C-30 group with a concentration of 100μmol/L was shown to inhibit the biofilm formation by Streptococcus mutans significantly（P0.05）.The time of magnetic beads immobilized and the value of BFI were both different between the 100μmol/L furanone C-30 group and the control groups.Conclusions：Quorum sensing inhibitor furanone C-30 in the concentration of 100μmol/L can inhibit the biofilm formation of Streptococcus mutans.It may direct a new way for the prevention and therapy of caries.

关 键 词：变异链球菌 生物膜 呋喃C-30 

分 类 号：R780.2[医药卫生—口腔医学]