地衣芽胞杆菌WX-02补糖发酵聚γ-谷氨酸的工艺优化  

Optimization of glucose fed-batch culture in poly(γ-glutamic acid) fermentation by Bacillus licheniformis WX-02

作　　者：胡丽芳[1] 李欣[1] 冀志霞[1] 陈守文[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2012年第3期287-292,共6页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30970036);农业科技成果转化资金项目(2010GB23600662)

摘　　要：以地衣芽胞杆菌(Bacillus licheniformis)WX-02为研究对象,在摇瓶发酵中得到优化的葡萄糖补料方案,即初始葡萄糖质量浓度为70g/L时,发酵至15h一次性补加15g/L葡萄糖。将此补料方案在3L发酵罐上进行验证,结果表明:发酵生物量和聚γ-谷氨酸产量最大值分别为3.9g/L和44.5g/L,与对照相比分别提高56%和50%;谷氨酸利用率达到46%,而对照中仅为30%。通过补糖可以提高生物量,提高对谷氨酸的利用率,从而提高聚γ-谷氨酸发酵水平。Through flask-shaking fed-batch fermentation,the optimum system of glucose feeding for poly（γ-glutamic acid）（γ-PGA） fermentation by Bacillus licheniformis WX-02 was summerized as follows：the initial concentration of glucose was 70.0 g/L,then 15.0 g/L of glucose was added at 15th hour of fermentation carried out in a 3.0 L fermentor.The results showed that the biomass and the yield of γ-PGA were 3.9 g/L and 44.5 g/L,increasing 56% and 50% compared with the control.The utilization rate of glutamic acid in the end of fermentation was 46%,while that of control was 30%.Feeding glucose at the 15th hour of fermentation process could increase biomass,enhance utilization of glutamic acid and finally increase the γ-PGA yield by B.licheniformis WX-02.

关 键 词：地衣芽胞杆菌 聚Γ-谷氨酸 补糖发酵 生物量 产量 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]