小鼠大脑中动脉栓塞模型的改良及评价  

Modification of Generating Middle Cerebral Artery Occlusion Model in Mice

作　　者：唐颖馨[1] 刘敏珍[1] 丁凤菲[1] 张强[1] 潘邓记[1] 王伟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《神经损伤与功能重建》2012年第2期79-83,共5页Neural Injury and Functional Reconstruction

基　　金：国家杰出青年基金项目(No.30725019)

摘　　要：目的:对小鼠大脑中动脉栓塞(MCAO)模型进行改良并评价。方法:C57BL/6J小鼠50只,随机分为假手术组10只,MCAO组40只;改良线栓法制作小鼠MCAO模型;术后24h行神经功能评分,TTC染色测脑梗死体积;术后30d,水迷宫测试小鼠远期学习和记忆能力;术后90d,头部MRI观察梗死病灶及脑组织变化。结果:造模成功率为67.5%;MCAO组小鼠神经功能评分为(2.36±0.21)分,相对梗死体积为(25.36±3.65)%;MCAO组小鼠逃避潜伏期明显长于假手术组,穿越原平台位置次数明显少于假手术组(P<0.05);小鼠头部MRI T2加权像可见缺血侧皮质和/或皮质下梗死病灶。结论:线栓法是简单、可靠的制作小鼠MCAO模型的方法。Objective： To modify the method to generate middle cerebral artery occlusion（MCAO） model in mice.Methods： Fifty C57BL/6J mice were randomly divided into sham group（n=10） and MCAO group（n=40）.The mice in MCAO group were used to establish the MCAO model by intraluminal occlusion using monofilament.Twenty-four hours after operation,the neurologic function was evaluated and the infarct volume was calculated by TTC staining.Thirty days after operation,Morris water maze test was carried out to investigate spatial learning and memory of the mice.Ninety days after operation,head MRI scan was conducted.Results： In the MCAO group,the satisfactory MCAO model was successfully generated in 67.5% out of the operated mice.The neurological deficit score was（2.36±0.21） and the relative infarct volume was（25.36±3.65） % in the MCAO group.The mice in the sham group performed better in water maze task than those in the MCAO group.T2-weighted MRI imagines showed infarct clearly.Conclusion： Intraluminal occlusion of MCA using monofilament is a kind of simple and reliable method to establish mouse MCAO model.

关 键 词：小鼠 大脑中动脉栓塞 动物模型 

分 类 号：R741[医药卫生—神经病学与精神病学] R743[医药卫生—临床医学]