肿瘤坏死因子-α对人肺癌A549细胞的放疗增敏作用  

Tumor necrosis factor-α enhances radiosensitivity of A549 cells

作　　者：夏晖[1] 于长海[1] 张宜明[1] 俞建琦[1] 李捷[1] 张文[1] 李英杰[1] 

机构地区：[1]中国人民解放军总医院第一附属医院胸心外科,北京100048

出　　处：《南方医科大学学报》2012年第4期565-568,共4页Journal of Southern Medical University

基　　金：首都临床特色应用研究基金(D101100050010024)

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)在人肺癌A549细胞放射治疗中的增敏作用。方法单独γ射线辐照细胞或联合应用TNF-α处理细胞,通过MTT法检测人肺癌A549细胞存活生长曲线,采用流式细胞术分别检测细胞凋亡和细胞周期的变化,并利用Western blot法分析细胞中Caspase-3蛋白表达的变化。结果与单独γ射线作用相比,TNF-α和γ射线联合应用能明显抑制人肺癌A549细胞的生长,同时诱导和促进A549细胞的凋亡发生,调整细胞周期的变化,这一过程与细胞中Caspase-3蛋白表达的增加相关。结论 TNF-α可以增加人肺癌A549细胞的放疗敏感性,提高γ射线对人肺癌A549细胞的放射治疗效果。Objective To assess the effects of tumor necrosis factor-α（TNF-α） in enhancing the radiosensitivity of lung cancer cells in vitro.Methods A549 cells were exposed to γ-ray with or without TNF-α treatment.MTT assay was used to evaluate the cell viability,and flow cytometry was performed to assess the cell apoptosis.Western blotting was used to observe the expression of caspase-3 protein in the exposed cells.Results Compared with the exposed cells without TNF-α treatment,the cells treated with TNF-α showed significantly suppressed cell proliferation,increased the cell apoptosis,altered cell cycle,and increased caspase-3 protein expression after γ-ray exposure.Conclusion TNF-α can enhance the radiosensitivity of A549 cells to increase the efficiency of radiotherapy with γ-ray irradiation.

关 键 词：肿瘤坏死因子-Α 肺癌 放疗增敏 

分 类 号：R734.2[医药卫生—肿瘤]