6-羟基多巴胺致帕金森病模型大鼠步态的CatWalk定量分析  

Quantitative CatWalk analysis of gaits in rats with 6-hydroxydopamine-induced PD

作　　者：王军[1] 张旺明[1] 罗敏捷[1] 潘琪[1] 蔡颖谦[1] 姜晓丹[1] 徐如祥[1,2] 

机构地区：[1]南方医科大学珠江医院神经外科,广东神经外科研究所,广东省脑功能修复与再生重点实验室,广州510282 [2]北京军区总医院附属八一脑科医院,北京100047

出　　处：《中华神经医学杂志》2012年第4期364-368,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30872662）;广东省科技计划引导项目（2008A03102010）;国家临床重点建设专科资助项目

摘　　要：目的探讨对单侧注射6-羟基多巴胺（6-OHDA）致帕金森病模型大鼠步态进行CatWalk定量分析的可行性。方法24只雄性Wistar大鼠按完全随机数字表分为6-OHDA组fn=12）、假手术组（n=6）与对照组m=6），其中6-OHDA组和假手术组采用立体定向手术将6-OHDA溶液和等量生理盐水分别注入大鼠的左侧内侧纵束，对照组仅行麻醉处理。造模前1周训练大鼠并采集行为学数据基线值。造模后3d、1周、2周对大鼠依次进行圆柱实验，再将大鼠置于CatWalk自动步态分析仪中，记录其步态变化，离线分析相关数据。结果圆柱实验结果显示：造模后3d、1周、2周时．与理论值50％比较，6．0HDA组大鼠右前肢的使用比例均较左前肢明显下降，差异均有统计学意义（P〈0．051；对照组及假手术组大鼠右前肢使用比例无明显异常，差异无统计学意义（尸〉0．05）。CatWalk分析结果显示：对照组、假手术组大鼠造模后左、右肢体对比，各肢体的造模前、造模后对比中各指标比较差异均无统计学意义（P〉0．05）。6-OHDA组大鼠左、右肢体对比中，右前肢的最大接触面积、爪印长度、爪印宽度和爪印面积较左前肢增加，左后肢的最大接触面积较右后肢增加，差异均有统计学意义（P〈0．05）；6-OHDA组大鼠各肢体的造模前、造模后对比中，左前肢的爪印长度、爪印宽度和爪印面积较造模前有所下降，右前肢的爪印面积、爪印长度和最大接触面积较造模前下降．在左后肢中仅爪印宽度下降．右后肢的最大接触面积、爪印长度、爪印宽度和爪印面积均较造模前减少，差异均有统计学差异（P〈0．05）。结论CatWalk可对帕金森病模型大鼠步态的变化进行定量分析，其中最大接触面积可能是最佳参数之一，可为深入研究帕金森病提供有益参考。Objective To investigate the probability of quantitative analysis of parkinsonian gaits in unilateral 6-hydroxyl dopamine-lesioned rats. Methods A total of 24 male Wistar rats were assigned to control group （n=6）, sham-operated group （n=6） and 6-OHDA inducement group （n=12）. All subjects received anesthetization and the latter 2 groups further processed with injection of equivalent volumes of normal saline and 6-OHDA into the left medial longitudinal fasciculus on the operation day, respectively. The cylinder test and Catwalk analysis were applied successively 1 week before the operation for baseline value, and 3 d, 1 and 2 weeks after the operation. Results The cylinder test indicated that the proportion of using the right forelimbs was significantly decreased as compared with that using the left forelimbs in the 6-OHDA inducement group 3 d, 1 and 2 weeks after the operation （P〈0.05）, but no significant differences of using the right forelimbs were shown in the control and sham-operated counterpart （P〉0.05）. Catwalk analysis showed no significant differences either on the girdle comparisons of former/hinder limbs or on pre- and post-operation comparisons of individual limbs between control and sham-operated groups （P〉0.05）. In the 6-OHDA inducement group, max contact area （MCA）, paw length （PL）, paw width （PW） and paw area （PA） for fore girdle comparisons and MCA for hinder ones showed significant differences （P〈0.05）; all the 4 parameters of right hinder limb, the PL, PW and PA of left fore limb, the PA, PL and MCA of right fore limb, the PW of left hinder limb after the operation significantly decreased as compared with those before the operation （P〈0.05）. Conclusion It is applicable to detect changes in gaits of Parkinson＇s disease quantitatively with Catwalk analysis, especially in terms of MCA, adding new tool for further study.

关 键 词：帕金森病 模型 动物 步态 CatWalk分析 6-羟基多巴胺 

分 类 号：R742.5[医药卫生—神经病学与精神病学]