一氧化氮及两种细胞因子在分泌性中耳炎中耳积液中的表达及意义  

Nitric oxide and cytokines in otitis media with effusion

作　　者：林国武[1] 黄维国 姜鸿彦[2] 王锦玲[2] 

机构地区：[1]广州武警广东总队医院耳鼻咽喉科,510507 [2]西安第四军医大学西京医院耳鼻咽喉科,710032

出　　处：《中华耳鼻咽喉科杂志》2000年第1期23-25,共3页Chinese Journal of Otorhinolaryngology

基　　金：国家自然科学基金!( 3 92 70 710 )

摘　　要：目的　探讨一氧化氮 (NO)和白细胞介素 8(interleukin 8,IL 8)、肿瘤坏死因子 α(tumornecrosisfactor α ,TNF α)在分泌性中耳炎发生和转归中的作用。方法　检测 5 8例 (80耳 )分泌性中耳炎患者血浆和中耳积液中NO、IL 8及TNF α ,并用 2 3例健康人血浆作对照。结果　NO、IL 8及TNF α在中耳积液中阳性表达率分别为 1 0 0 %、82 2 %和 71 4 % ;患者组三者的血浆含量较对照组高 (P <0 0 5 ) ,中耳积液的含量较血浆中高 (P <0 0 1 )。病程短者IL 8含量高 (P <0 0 1 ) ;病程长者NO及TNF α含量高 (P <0 0 5 )。浆液性积液组IL 8含量高 (P <0 0 1 ) ;粘液性积液组NO及TNF α的含量高(P <0 0 5 )。NO含量与TNF α含量呈正相关。结论　NO、IL 8、TNF α可能是中耳积液形成的诸多介质中的 3种 ;IL 8在疾病早期参与机体的防御反应 ,促进浆液性中耳积液产生 ;NO和TNF α与疾病的持续状态相关 ,并参与中耳积液中粘蛋白的分泌。Objective To study the role of cytokines and nitric oxide (NO) in the pathogenesis of secretory otitis media (SOM) Methods The content of NO, interleukin 8 (IL 8) and tumor necrosis factor alpha (TNF α)in blood plasma and middle ear effusion (MEE) were measured in 58 (80ears) patients with secretory otitis media and in 23 normal subjects. respectively. Results NO, IL 8 and TNF α were found in 100%, 82 2% and 71 4% of MEE respectively NO, IL 8 or TNF α of blood plasma in the SOM group was higher than those in the normal control group ( P <0 05) The concentration of NO, IL 8 or TNF α in MEE was remarkably higher than those in blood plasma ( P <0 01) It revealed that the shorter the course, the higher the concentration of IL 8 in MEE ( P <0 01), and the longer the course, the higher the concentration of NO or TNF α in MEE ( P <0 05) IL 8 concentration of MEE in serous fluid group was remarkably higher than that in the mucous fluid group ( P <0 01), and the concentration of NO or TNF α of MEE in the mucous fluid group was higher than that in the serous fluid group ( P <0 05) NO level of MEE was positively correlated with TNF α( r =0 696 P <0 01). Conclusion NO、IL 8 and TNF α might be important mediators in MEE secretion in SOM IL 8 might participate in the defensive reaction of organism during the early stages of SOM, and play a role in serous effusion secretion NO and TNF α might be closely related to the persistence of SOM, and might be a mediator of mucin secretion in SOM

关 键 词：中耳炎 一氧化氮 IL-8 TNFΑ 分泌性 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]