骨髓瘤细胞对血管内皮细胞的促生长作用及机制  

The biological function and mechanism of Myeloma cells on endothelial cells

作　　者：董晓辉[1] 黄红铭[1] 徐瑞容[1] 薛英姿[1] 丁润生[2] 戚菁[1] 

机构地区：[1]南通大学附属医院血液科,江苏226001 [2]南通大学附属医院外科综合实验室,江苏226001

出　　处：《交通医学》2012年第1期29-32,共4页Medical Journal of Communications

基　　金：南通市社会发展指导计划资助项目(S2009026)

摘　　要：目的:研究多发性骨髓瘤细胞对人内皮细胞增殖、迁移、血管生成及对内皮细胞AKT、pAKT蛋白水平的影响。方法:采用多发性骨髓瘤细胞株U266与人脐静脉内皮细胞(HUVEC)混合共培养;以同期单独培养的HUVEC为对照,用CCK-8、划痕试验检测共培养组和对照组HUVEC的增殖、迁移能力。观察在Matrigel基质中两组HUVEC管状结构生成的情况;Western blot方法检测共培养组和对照组HUVEC AKT、pAKT的蛋白表达水平。结果:与同期单独培养的HUVEC相比,共培养体系中的HUVEC细胞增殖明显增加,共培养组HUVEC迁移能力明显增强(125±21/视野vs.332±37/视野),共培养组HUVEC管状结构形成数量明显增加(小管数量分别为3.7±1.3/视野vs.8.3±2.7/视野)。共培养体系中HUVEC pAKT表达水平明显升高,AKT表达无明显变化。结论:骨髓瘤细胞能明显促进血管内皮细胞增殖、迁移和血管生成。多发性骨髓瘤促进HUVEC增殖、迁移和血管生成的作用与AKT信号通路有关。Objective ：To investigate the impact of human multiple myeloma cells U266 on human umbilical vein endothelial cells（HUVEC） proliferation,migration,angiogenesis,and the expression of AKT,pAKT.Methods：Multiple myeloma cells U266 and HUVEC were cultured as a mixed co-culture group to the same period HUVEC cultured alone as the control group to detect the proliferation of HUVEC in the two groups by CCK-8 and the migration of HUVEC and by scratch test to observe HUVEC angiogenesis in Matrigel matrix and to find the expression of AKT and pAKT in HUVEC of co-culture group by Western blot.Results：Compared with the control group,the number of HUVEC cells was markedly increased after co-culture.Migration of endothelial cells in co-culture group was significantly increased compared with that in the control group,the number being 125±21/HP and 332±37/HP.In the Matrigel matrix,the number of tube formation in endothelial cells of the co-culture group was significantly increased compared with that in the control group,the number being 3.7±1.3/HP and 8.3±1.3/HP.The expression of AKT and pAKT in HUVEC was significantly increased after co-cultured,but AKT protein expression was not statistically significant.Conclusion：Multiple myeloma cells can promote the HUVEC proliferation,migration and angiogenesis,showing that the promotion of multiple myeloma cells to HUVEC was related with the AKT signaling pathway.

关 键 词：多发性骨髓瘤 人脐静脉内皮细胞 血管新生 共培养 AKT蛋白 

分 类 号：R733.3[医药卫生—肿瘤]