青风藤中总生物碱的气相色谱-质谱联用分析  

Analysis of Total Alkaloids in Sinomenium acutum by Gas Chromatography-Mass Spectrometry

作　　者：吴振启[1] 郭剑明[2] 陈琴华[3] 李慧峰[1] 李伟亮[1] 张菊根[1] 王国民[2] 

机构地区：[1]复旦大学附属中山医院青浦分院泌尿外科,上海201700 [2]复旦大学中山医院泌尿外科,上海200032 [3]武汉大学药学院,430070

出　　处：《医药导报》2012年第4期495-497,共3页Herald of Medicine

摘　　要：目的采用索氏提取法对青风藤中总生物碱进行提取分离,并利用气相-质谱联用(GC-MS)方法,对其化学成分进行分析。方法采用90%无水乙醇提取,盐酸酸化后,氨水碱化后三氯甲烷萃取,利用GC-MS对总生物碱中的化学成分进行分离分析,并对其中的15种化学成分进行鉴定。结果该方法提取时青藤碱含量高,达到80.18%,安贝灵碱3.44%,光千金藤碱3.05%。采用峰面积归一化法计算各成分相对百分含量,所鉴定的成分占总流出峰面积的95.1%。结论实验结果为进一步开发研究青风藤中生物碱提供依据。Objective To extract total alkaloids of Sinomenium acutum by Soxhlet extractor and analyze them by gas chromatography-mass spectrometry（GC-MS）.Methods The Sinomenium acutum was extracted by 90% alcohol,acidified by hydrochloric acid,alkalinized by ammonia water,and then extracted by chloroform.Fifteen peaks were separated and identified by GC-MS.Results The relative content of sinomenine,ambelline and stepharine was up to 80.18%,3.44% and 3.05%,respectively.The compounds were quantitatively determined by normalization method,and the identified compounds represented 95.1% of total GC peak area.Conclusion The results provide important foundation to further study and exploit Sinomenium acutum.

关 键 词：青风藤 青藤碱 总生物碱 气相色谱-质谱联用 

分 类 号：R927[医药卫生—药学]