烟雾暴露大鼠肺血管重塑及其与过氧化物酶体增殖物激活受体γ表达的关系  

Correlation of expression of PPAR-γ in pulmonary vascular and remodeling of pulmonary vascular induced by cigarette smoke exposure in rats

作　　者：秦艳艳[1] 杜永成[2] 胡晓芸[3] 邢爱萍[3] 赵瑛娟[3] 

机构地区：[1]山西医科大学,太原030001 [2]山西省人民医院呼吸科,太原030001 [3]山西医科大学第一医院呼吸科,太原030001

出　　处：《国际呼吸杂志》2012年第7期513-516,共4页International Journal of Respiration

摘　　要：目的探讨烟雾暴露对大鼠肺血管过氧化物酶体增殖物激活受体γ（PPAR-γ）表达的影响及其在肺血管重塑中的作用。方法雄性SD大鼠30只，随机分为对照组、烟雾暴露6周组、烟雾暴露12周组，建立大鼠烟雾暴露模型。HE染色观察各组大鼠肺组织形态学改变，测量肺血管管壁厚度与血管外径的比值（wT％）及管壁面积与血管总面积的比值（WA％）；免疫组织化学染色检测肺血管PPAR-y的表达量，并分析各指标的相关性。结果①烟雾暴露6周组wT％及wA％[（45．61±2．43）％及（59．69±12．59）％]与烟雾暴露12周组[（54．23±6．22）％及（76．26±8．36）％]均高于对照组[（a2．89±7．65）％及（33．05±14．84）％]，差异均有统计学意义（P值均〈0．05）；烟雾暴露12周组wT％及WA％高于烟雾暴露6周组，差异均有统计学意义（P值均〈O．05）；②烟雾暴露6周组与烟雾暴露12周组PPAR-γ蛋白表达[（0．48±0．08），（0．37±0．07）]均低于对照组（O．51±0．07），差异均有统计学意义（P值均〈O．05）；烟雾暴露12周组PPAR-γ蛋白表达低于烟雾暴露6周组，差异有统计学意义（P〈O．05）；③肺血管PPAR-γ与wT％及WA％呈负相关（r值分别为-0．715和-0．683，P值均〈0．05）。结论烟雾暴露可下调大鼠肺血管PPAR-γ的表达，参与肺血管重塑。Objective To investigate the expression variation of peroxisome proliferator-activated receptor y （PPAR-y） in pulmonary vasculature in rats exposed to cigarette smoke and its role in pulmonary vascular remodeling. Methods 30 male SD rats were randomly divided into a control group, a smoke exposure for 6 weeks group, a smoke exposure for 12 weeks group, with 1O rats in each group. The ratio of pulmonary vascular wall thickness/vascular external diameter （WT%）and the ratio of pulmonary vascular wall area/total pulmonary vascular area （WA%）were measured by the image analysis system. The expression of PPAR-y protein in pulmonary vascular of each group was detected by Immunohistoehemistry. Results ①WT% and WA% increased significantly in the smoke exposure for 6 weeks group [-（45.61±2.43）%, （59.69±12.59）%] and smoke exposure for 12 weeks group [（54.23±6.22）%,（76. 26±8. 36）%] compared to those of control group [（32.89±7.65） %,（33.05±14.84）%] （all P %0.05）. The WT% and WA% in the smoke exposure for 12 weeks group increased significantly compared to those of smoke exposure for 6 weeks group （all P〈0.05）. ②The expression of PPAR-3＇ protein in pulmonary vascular decreased significantly in the smoke exposure for 6 weeks group （0.48±0.08）and the smoke exposure for 12 weeks group （0.37±0.07） compared to control group （0.51±0.07）（all P〈0.05）. The expression of PPAR-y protein decreased in the smoke exposure for 12 weeks group compared to the smoke exposure for 6 weeks group （ P〈0.05）. ③The expression of PPAR-γ protein in pulmonary vascular was respectively negatively correlated with WT% and WA% （ r =--0. 715 and 0. 683） （all P 〈~0.05）. Conclusions By down-regulating the expression of PPAR y protein in pulmonary vascular, cigarette smoke exposure induces the remodeling of pulmonary vascular.

关 键 词：烟雾暴露 重塑 过氧化物酶体增殖物激活受体Γ 

分 类 号：R363[医药卫生—病理学]