两种麦类土传花叶病毒外壳蛋白叶绿体离体跨膜运输  

Analysis on the Import of Coat Proteins of Chinese Wheat Mosaic Virus and Barley Yellow Mosaic Virus into Intact Chloroplasts in Vitro

作　　者：任春梅[1] 程兆榜[1] 魏利辉[1] 范永坚[1] 周益军[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014

出　　处：《麦类作物学报》2012年第1期163-167,共5页Journal of Triticeae Crops

基　　金：农业部产业体系项目(nycytx-03);农业部行业专项(nyhyzx07-051);江苏省农科院基金项目(6510703)

摘　　要：根据Banerjees等(1992)的方法,建立了中国小麦花叶病毒(CWMV)和大麦黄花叶病毒(BaYMV)外壳蛋白(CP)叶绿体离体跨膜运输体系,研究了孵育时间、CP浓度对跨膜运输效率的影响。结果表明,CWMV-CP和BaYMV-CP可分别快速进入离体的小麦与大麦叶绿体中,其跨膜运输所需的孵育时间均最低为5min,孵育时间超过15min后进入叶绿体中CP的量不受影响;加入跨膜体系中CP的浓度与跨膜后进入叶绿体的CP浓度呈正相关,能够实现跨膜的最低CWMV-CP和BaYMV-CP浓度分别为4.2和37.8μg.mL-1。Referred to Banerjee＇s method, the systems of importing the coat proteins of Chinese wheat mosaic virus and Barley yellow mosaic virus （CWMV CP and BaYMV-CP） into intact chloroplasts in vitro were established, the effects of incubation duration, the concentration of CWMV-CP and BaYMV-CP on import efficiency were studied. The results showed that both CWMV-CP and BaYMV- CP was able to rapidly import into isolated chloroplasts of wheat and barley. In the test, the lowest incubation duration of CWMV-CP and BaYMV-CP importing into the chloroplasts were both 5 min, the amounts of CWMV-CP and BaYMV-CP imported into the chloroplasts were not increased with the extension of incubation time after 15 min. Besides, the relation between the amounts of CWMV-CP and BaYMV-CP imported into the chloroplasts and the amounts of CWMV-CP and BaYMV-CP added in the import system was significantly positive, the lowest concentrations of CWMV CP and BaYMV- CP in the import system was 4.2 and 37.8 μg. mL^-1 , respectively. The establishment of CWMV-CP and BaYMV-CP importing into intact chloroplasts in vitro could supply foundation for studying the pathogenesis of CWMV and BaYMV.

关 键 词：中国小麦花叶病毒 大麦黄花叶病毒 外壳蛋白 跨膜运输 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]