TGF-β2/Smad2在人脑胶质瘤组织中的表达及意义  

Expression of transforming growth factor beta-2/Smad2 in human glioma and its significance

作　　者：曹泽[1] 金贵善[1] 刘福生[1] 谢坚[1] 

机构地区：[1]北京市神经外科研究所 首都医科大学附属北京天坛医院神经外科,100050

出　　处：《中华临床医师杂志（电子版）》2012年第6期43-46,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：北京市自然科学基金(7102027)

摘　　要：目的研究转化生长因子β(TGF-β)信号传导通路中TGF-β2/Smad2在人脑胶质瘤组织中的表达和意义。方法收集人脑胶质瘤组织样本59例,免疫组织化学法检测TGF-β2、Smad2、磷酸化Smad2(P-Smad2)及Ki-67蛋白在其中表达情况,反转录聚合酶链反应(RT-PCR)检测其中Smad2 mRNA的表达。结果 TGF-β2、Smad2、P-Smad2及Ki67蛋白与人脑胶质瘤病理级别呈正相关(P<0.01),各级别均数比较差异均有统计学意义(P<0.05);TGF-β2、Smad2、P-Smad2蛋白表达之间亦呈正相关(P<0.01);TGF-β2、Smad2及P-Smad2蛋白表达均与增殖指标Ki-67蛋白表达呈正相关(P<0.01)。Smad2 mRNA表达与Smad2和P-Smad2蛋白表达呈正相关(P<0.05)。结论 TGF-β2/Smad2蛋白表达随肿瘤病理级别增高表达增强,与胶质瘤的恶性进展有关。Objective To investigate the expression and significance of transforming growth factor beta-2（TGF-β2）/Smad2 in the TGF-β/Smads signaling pathway in human gliomas.Methods Immunohistochemistry was performed to analyze the expression of TGF-β2,Smad2,phosphorylated-Smad2（P-Smad2）and Ki-67 in 59 patient-derived specimens of human gliomas.Reverse transcription polymerase chain reaction（RT-PCR）was used to analyze the level of Smad2 mRNA.Results The expression of TGF-β2,Smad2,P-Smad2 and Ki-67 was positively correlated with the pathological grades and with each other（P0.01）.There was statistically significant difference of TGF-β2,P-Smad2 and P-Smad2 expression between the pathological grades（P0.05）.The expression of TGF-β2,Smad2 and P-Smad2 was positively correlated with each other（P0.01）.The expression of TGF-β2,Smad2 and P-Smad2 was positively correlated with expression of Ki-67（P0.01）.The Smad2 mRNA level was positively correlated with the expression of Smad2 and P-Smad2（P0.05）.Conclusions The expression of TGF-β2 and Smad2 which was positively correlated with the pathological grades may promote the proliferation and the malignant progression.

关 键 词：神经胶质瘤 转化生长因子Β2 Smad2蛋白质 免疫组织化学 

分 类 号：R739.4[医药卫生—肿瘤]