双调控溶瘤腺病毒携带SEA基因靶向鼠膀胱癌的表达  

Expression of targeting to the mouse bladder cancer by double-promoter regulated adenovirus carrying SEA gene

作　　者：陈猛[1] 郝林[2] 史振铎[2] 张辉[1] 董洋[1] 韩从辉[2] 

机构地区：[1]东南大学医学院,江苏南京210009 [2]东南大学医学院附属徐州医院泌尿外科,江苏徐州221009

出　　处：《东南大学学报（医学版）》2012年第2期166-169,共4页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(30973443);江苏省自然科学基金资助项目(BK2009085)

摘　　要：目的:研究hTERT和HIF启动子双调控的溶瘤腺病毒能否感染鼠膀胱癌细胞并表达治疗基因,为动物实验提供依据。方法:以不同浓度溶瘤腺病毒感染鼠膀胱癌细胞,生物倒置显微镜下动态观察细胞形态变化,RT-PCR检测SEA在细胞内mRNA表达,Western blot检测SEA蛋白表达。结果:镜下可见细胞感染腺病毒并最终裂解,RT-PCR和Western blot分别检测到实验组mRNA和蛋白的表达。结论:携带SEA基因的hTERT/HIF双调控溶瘤腺病毒可在鼠膀胱癌细胞内复制增殖并表达SEA基因,可用于治疗鼠膀胱癌的动物实验研究。Objective：To study human telomerase reverse transcriptase（hTERT）/hypoxia-inducible factor（HIF）-promoter regulated adenovirus whether can infect the mouse bladder cancer and express theoretical gene,providing evidence for the animal experiment.Methods：The cells of the mouse bladder cancer were infected with different concentration of adenovirus;The morphologic changes of cells were observed under the microscope;Staphylococcal enterotoxins A（SEA） in the nucleic acid expressions in tumor cells were detected by real-time quatiative polymerase chain reaction（RT-PCR） and protein expressions of SEA were detected by Western blot.Results：The cells were infected by adenovirus and disrupted,finally.The mRNA and protein of SEA were successfully expressed.Conclusion：The adenovirus can replicate and proliferate in the mouse bladder cancer cells and can be used in treating the mouse bladder cancer in vivo.

关 键 词：溶瘤腺病毒 膀胱癌 金黄色葡萄球菌肠毒素A 人端粒酶逆转录酶 缺氧诱导因子 

分 类 号：R737.1[医药卫生—肿瘤] R73-354[医药卫生—临床医学]