miR-214对胃癌细胞SGC7901侵袭和转移能力的影响  

Enhancement effects of miR-214 on invasive and metastatic ability of gasric cancer cell line SGC7901

作　　者：赵雪琰[1] 郑长黎[1] 

机构地区：[1]中南大学基础医学院病理学系,湖南长沙410013

出　　处：《中国普通外科杂志》2012年第4期421-426,共6页China Journal of General Surgery

摘　　要：目的:探讨miR-214对胃癌细胞侵袭和转移能力的影响及其可能作用机制。方法:应用miRanda,TarBase v.5c靶基因预测网站分析miR-214与PTEN mRNA的可能结合位点,分别将miR-214模拟物(mimics),拮抗物(inhibitors)和无关序列转染到SGC7901细胞株,以空白转染组为阴性对照,用Western blot的方法比较各组细胞PTEN蛋白的表达,用Transwell侵袭小室检测各组细胞侵袭能力的变化。结果:Western blot结果显示,miR-214 mimics转染组的SGC7901细胞中PTEN蛋白表达较其余各组显著降低(均P<0.05),Transwell侵袭小室检测结果表明,miR-214 mimics转染组的细胞侵袭细胞数明显多于其余各组(均P<0.05)。结论:miR-214能促进胃癌细胞侵袭和转移,其机制可能与抑制PTEN的表达有关。Objective： To investigate the effect ofmiR-214 on the invasive and metastatic ability of gastric cancer ceils and its possible mechanism. Methods： The potential binding sites of PTEN （phosphatase and tensin homolog deleted on chromosome ten） for miR-214 were analyzed at miRNA target-gene prediction websites： miRanda and TarBase v.Sc. The SGC7901 cells were transfected with miR-214 mimic, inhibitor or non-targeting sequence, and cells transfected with the empty vector were used as negtive control. After the above treatments, the PTEN protein expression of the cells was detected by Western blot analysis and the invasive ability of the cells was examined by Transwell chamber assay. Results： Western blot showed that PTEN protein expression in SGC7901 cells group transfected with miR-214 mimic was significantly decreased compared with the cells of other treatment groups （all P〈0.05）. Transwell assay showed that the invading cell numbers of SGC7901 cells group transfected with miR-214 mimic were significantly more than those of the cells of other treatment groups （all P〈0.05）. Conclusion： miR-214 can enhance the invasion and metastasis of gastric cancer cells and this effect may be related to its action in inhibiting PTEN expression.

关 键 词：胃肿瘤/病理学 MICRORNA miR-214 肿瘤侵袭 

分 类 号：R735.2[医药卫生—肿瘤]