苦参碱对白色念珠菌细胞周期的影响  

Effect of matrine on the cell cycle of Candida albicans

作　　者：吴岚[1] 施琳俊[1] 薛婷君[1] 周曾同[1] 周永梅[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院,上海200011

出　　处：《临床口腔医学杂志》2012年第4期200-202,共3页Journal of Clinical Stomatology

基　　金：上海市自然科学基金资助项目(11ZR1420400)

摘　　要：目的:观察苦参碱对白色念珠菌标准菌株ATCC76615周期的影响。方法:将白色念珠菌标准菌株ATCC76615培养在含不同药物浓度的YPD培养基中,培养6、12和24 h,流式细胞仪检测细胞生长周期、DNA荧光强度和细胞体积大小。结果:白色念珠菌标准菌株ATCC76615在苦参碱作用24 h后,生长受到抑制,并且药物浓度越高生长抑制越明显;白色念珠菌标准菌株ATCC76615的细胞体积随着苦参碱浓度的增高而逐渐变小。结论:苦参碱对白色念珠菌标准菌株ATCC76615细胞生长周期有抑制作用;流式细胞仪可用于抗真菌药物敏感性测定。Objective：To determine the effects of matrine on the cell cycle of Candida albicans （ATCC76615）. Method： After Candida albicans （ATCC76615）were incubated for 6 hours, 12 hours and 48 hours in YPD, which contained serial concentrations of matrine, the cel 1 cycle, fluorescent intensity and size of cell volume were detected by flow cytometry （FCM）. Result：The cell division was inhibited when Candida albicans （ATCC76615）were incubated with matrine for 24 hours. The size of Candida albicans＇ cell volume, cell diameter and particle size changed more as the concentrations of matrine increased. Conclusion： Matrine could inhibit the cell cycle of Candida albicans in different range.FCM could be used as a method in determining the susceptibility of antifungal agents.

关 键 词：白色念珠菌 苦参碱 细胞周期 流式细胞术 

分 类 号：R780.2[医药卫生—口腔医学]