微囊藻毒素-RR高效降解菌的分离鉴定及降解特性  

Isolation and Identification of a Microcystin-RR Degrading Bacterium and Its Biodegradation Characteristics

作　　者：刘凯英[1] 薛罡[1] 程起跃[1] 石为民[1] 叶文婷[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620

出　　处：《环境科学与技术》2012年第4期22-26,共5页Environmental Science & Technology

基　　金：教育部新世纪优秀人才计划(NECT-07-0175);上海市基础研究重点项目(08JC1400500);上海市自然科学基金(10ZR1401100);教育部博士点基金新教师项目(200802551001);长江水环境教育部重点实验室开放基金项目(YRWEF08003)

摘　　要：从蓝藻爆发期的上海市淀山湖表层水体中筛选分离降解微囊藻毒素-RR(MC-RR)的细菌,研究其降解特性。根据分离菌株的细胞形态结构、生理生化特征及其16S rDNA序列分析鉴定降解菌,高效液相色谱法测定该菌株降解MC-RR的能力。分离菌株DHU-38(GenBank序列登录号为HM047515)属荧光假单胞菌(Pseudomonas fluorescens)。微囊藻毒素降解实验结果表明,该菌株能在以MC-RR为唯一碳源、氮源的无机盐培养基中生长,6 d内可将初始质量浓度为20 mg/L的MC-RR降解为6.23 mg/L,降解效率达到69%。菌株DHU-38的最适生长温度是30℃,最适生长pH值为7.0。酵母粉、蛋白胨、葡萄糖等营养物质可以明显促进菌株对MC-RR的降解效率,尤其是加入100 mg/L酵母粉后,6 d降解率达到89.6%。A strain of Microcystin-RR （MC-RR）degrading bacterium was isolated from the surface waters in Dianshanhu, Shanghai and identified based on its morphology, physiology and phylogenetic analysis of 16S rDNA sequence. The degradation ability of this strain was evaluated with high performance liquid chromatography. Strain DHU-38 was identified to be closely related to Pseudomonas fluorescens（GenBank accession number is HM047515）. DHU-38 could grow on mineral salt medium supplied with MC-RR as sole carbon and nitrogen source. Initial MC-RR of 20 mg/L was degraded to 6.23 mg/L in 6 days and the average biodegradation rate is 69%. The optimum condition of degrading bacterium DHU-38 was at 30℃, pH 7.0. Yeast extract, peptone and glucose could significantly enhance the degradation rate of MC-RR. Especially, strain DHU-38 could degrade MC-RR by 89.6% in liquid mineral salt medium with 100 mg/L yeast extract.

关 键 词：微囊藻毒素-RR 生物降解 荧光假单胞菌 

分 类 号：X172[环境科学与工程—环境科学]