营养条件对污水中小球藻生长及产能效应的影响  

Effects of Nutritional Conditions on the Growth and Energy Efficiency of Chlorella Vulgaris in the Wastewater

作　　者：马剑敏[1,2] 苏秀燕[1] 马顷[1] 王程丽[1] 董文静[1] 靳同霞[1] 马帅[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南省环境污染控制重点实验室,河南新乡453007

出　　处：《环境科学与技术》2012年第4期27-32,81,共7页Environmental Science & Technology

基　　金：河南省科技厅重点攻关项目资助(102102210182)

摘　　要：以过滤的富营养化的鱼塘废水为培养液,添加外源的碳、氮、磷元素,考察污水中不同的外源无机碳浓度、总氮浓度、总磷浓度对小球藻(Chlorella vulgaris)的生长、油脂含量和烃类含量的影响。在25℃、光照强度为4 500 Lux、光暗比为12L:12D的条件下培养10 d。单因子方差分析和多重比较结果表明:(1)以Na2CO3做碳源,小球藻生物量和烃类含量在外源无机碳浓度为6 mg/L时最高,油脂含量在外源无机碳浓度为12 mg/L最高。(2)以KNO3做氮源,小球藻生物量在总氮浓度为25 mg/L时最高,油脂含量在总氮浓度为15 mg/L时最高,烃含量在总氮浓度为20 mg/L时最高。(3)以KH2PO4做磷源,小球藻生物量和烃类含量在总磷浓度为2 mg/L时最高,油脂含量在总磷浓度为1.5 mg/L时最高。With eutrophication wastewater of ponds filtered as culture medium, adding carbon source, nitrogen source and phosphorus source, the influences of different concentrations of inorganic carbon, total nitrogen and total phosphorus of wastewater on biomass, lipid content and hydrocarbon content of Chlorella vulgaris were studied. The culture had grown at the temperature of 25℃, the light intensity of 4,500 Lux, the photoperiod of 12L ： 12D for ten days. The results of one way anova and multiple comparison showed that with Na2COa as carbon source, biomass and hydrocarbon content of Chlorella vulgaris were the highest at the inorganic carbon concentration of 6 mg/L when the highest lipid content was at the inorganic carbon concentration of 12 mg/L. With KNO3 as nitrogen source, the biomass of Chlorella vulgaris was the highest at the total nitrogen concentration of 25 mg/L; the highest lipid content was at the total nitrogen concentration of 15 mg/L; the highest hydrocarbon content was at the total nitrogen concentration of 20 mg/L. With KH2PO4 as phosphorus source, biomass and hydrocarbon content of Chlorella vulgaris were the highest at the total phosphate concentration of 2 mg/L when the highest lipid content was at the total phosphorus concentration of 1.5 mg/L.

关 键 词：小球藻 污水 油脂 烃 无机碳 总氮 总磷 

分 类 号：X173[环境科学与工程—环境科学]