杂交中稻广两优476品种与纯度的鉴定  

The Variety and Purity Indentification of Hybrid Rice Guangliangyou 476

作　　者：杜雪树[1] 戚华雄[1] 夏明元[1] 

机构地区：[1]湖北省农业科学院粮食作物研究所,武汉430064

出　　处：《湖北农业科学》2012年第6期1254-1256,1262,共4页Hubei Agricultural Sciences

基　　金：科技部农业科技成果转化资金项目(2010GB2D100297)

摘　　要：为辨别杂交中稻广两优476种子真伪并鉴定其纯度,利用农业部《水稻品种鉴定DNA指纹方法》(NY/T 1433—2007)中推荐的24对引物进行PCR,使用聚丙烯酰胺凝胶电泳对广两优476及其亲本,以及另外几个育种常用亲本材料进行了多态性筛选,并利用筛选所得引物的SSR标记对广两优476进行了种子纯度分析。结果表明,这24对引物在几个常用亲本及广两优476间具有良好的多态性,可用于构建广两优476的DNA指纹图谱。同时,从中筛选得到标记RM209和RM17的两对引物在广两优476父母本间有显著多态性,其中RM209的引物的PCR产物在2%琼脂糖凝胶电泳条件下分辨效果最好,可用于广两优476杂交种子的纯度鉴定。2009年冬在实验室使用RM209鉴定广两优476杂交种子纯度,结果为98.6%,与2010年春在海南田间种植鉴定纯度98.3%相符。24 SSR markers recommended by China National Rice Research Institute were screened; and the markers showing polymorphism among hybrid rice parents of Guangliangyou 476 and other popular eultivars were used for purity test of Guan- gliangyou 476 seeds. It was showed that the SSR markers were with great polymorphism among the parents and Guangliangyou 476, thus were suitable for building of fingerprint of Guangliangyou 476. 2 SSR markers, RM209 and RM17, were detected with significant polymorphism between R476 and Guangzhan63S; moreover, bands of RM209 were well departed by 2% agarose gel electrophoresis, making it suitable for the purity test of Guangliangyou 476 seeds. The laboratory tested purity of Guangliangyou 476 hybrid seed in winter 2009 using RM209 was 98.6%, which was consistent with the filed tested result in Hainan spring 2010（98.3%）.

关 键 词：SSR标记 杂交水稻 广两优476 品种鉴定 纯度鉴定 

分 类 号：S511.32[农业科学—作物学] S339.31