GIi1和Foxm1在胃癌中的表达及其临床意义  

Expression of Glil and Foxml in Gastric Cancer and Its Clinical Significance

作　　者：冯亚光[1] 魏正强[1] 曾绍兵[1] 潘屹[1] 

机构地区：[1]重庆医科大学附属第一医院胃肠外科,重庆市400016

出　　处：《中国肿瘤临床》2012年第7期377-381,共5页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨Ihedgehog信号通路分子Glil与转录因子Foxml在胃癌中的表达及其与临床病理特征的关系。方法:收集临床胃癌病理标本70例及其相应癌旁正常组织30例,采用免疫组化SP法检测G1i1与Foxml蛋白在胃癌及正常组织中的表达,分析两者的相关性,RT-PCR检测两者mRNA在胃癌组织与正常组织的表达情况。结果:Glil和Foxml在正常胃粘膜组织中低表达或者不表达,Glil和Foxml在胃癌中的表达明显高于正常组织,差异具有统计学意义(P<0.05)。Gllil在胃癌中的表达与组织分化程度、淋巴结转移相关(P<0.05),而Foxml在胃癌中的表达仅与淋巴结转移相关(P<0.05),两者的表达与患者的性别、年龄、肿瘤的大小、浸润程度无显著相关(P>0.05)。且Glil和FoXnll在胃癌组织中的表达呈正相关(r=0.897,P<0.05)。结论:Glil和FoXnll在胃癌中的高表达可能与胃癌的发生发展有关,两者的正相关说明在胃癌的发生中两者有协同及相互调节的作用j两者联合检测可以成为胃癌检测、判断预后的指标,对新药物的开发及对胃癌化疗有一定的指导作用。Objective： This study evaluates the expression of Glil in Sonic hedgehog signal pathway and Foxml in gastric cancer, and elucidates the potential relationship of Glil and Foxml expression with clinical pathological characteristics. Methods： The expression of Glil and Foxml in 70 gastric carcinoma tissues and 30 non-cancerous stomach tissues was detected through immunohisto- chemistry and reverse transcriptase-polymerase chain reaction. The relationship between Glil and Foxml was also analyzed. Results： Glil and Foxml were either unexpressed or showed low expression in normal tissue. That is, Glil and Foxml expression was obviously higher in gastric cancer tissue than in normal tissue. The high expression of Glil mRNA and protein in gastric cancer was positively correlated with the differentiation of gastric cancer and lymph node metastasis （ P 〈 0.05 ）, whereas that of Foxml was only positively correlated with lymph node metastasis （ P 〈 0.05 ）. Glil and Foxml expression exhibited no correlation with patients＇ age, gender, tu- mor size, or depth of invasion （ P 〉 0.05 ）. A positive correlation was found between Gli 1 and Foxml expression （ r = 0.897; P 〈 0.05 ）. Conclusion： The overexpression of Gli 1 and Foxm 1 plays important roles in the pathogenesis of gastric cancer. In addition, the positive correlation between Gli 1 and Foxml proves their synergetic and mutual regulation in the occurrence and development of gastric cancer. The joint detection of Gli 1 and Foxm 1 may be of important clinical significance for tumor prognosis and may provide some experimental and clinical evidence for drug development and gastric carcinoma treatment.

关 键 词：胃癌Glil FOXM1 免疫组化 反转录-聚合酶链反应(RT-PCR) 

分 类 号：R735.2[医药卫生—肿瘤]