血必净注射液对家兔大动脉病变所致缺血／再灌注损伤的作用研究  

Effect of Xuebijing injection on rabbits ischemia/reperfusion injury induced by femoral arterial disease

作　　者：马杰飞[1] 宣丽真[1] 吴威[1] 诸杜明[1] 

机构地区：[1]复旦大学附属中山医院SICU,上海200032

出　　处：《中国危重病急救医学》2012年第4期233-236,共4页Chinese Critical Care Medicine

基　　金：天津市科技创新专项基金（06F22DSH00403）

摘　　要：目的探讨家兔缺血／再灌注（I／R）损伤模型建立后血液中丙二醛（MDA）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、天冬氨酸转氨酶（AST）、肌酐（cr）的变化，以及血必净注射液对急性I／R损伤的肝。肾保护作用。方法清洁级家兔60只，按随机数字表法分为6组：正常组，假手术组行同侧股动脉部位皮肤切开缝合，模型组以及血必净低、中、高剂量治疗组；建立I／R损伤模型（股动脉缺血4h后再通）后即刻及12、36、60h分别从耳缘静脉注射2ml／kg生理盐水或0．33、0．66、1．32g／kg血必净注射液。各组于术后6、12、24及72h分别抽血检测血清中MDA、IL-1β、TNF-α．、AST、Cr。结果模型组术后各时间点MDA、IL-1β、TNF-α．及术后72hAST、Cr均显著高于正常组和假手术组。与模型组比较，血必净各组随剂量增加各指标依次降低，血必净高剂量组6、12、24、72h血清MDA（Ixmo]JL）、IL-1β（ng／L）和TNF-α（g／L）均明显降低（MDA：9．74±3．71比12．35±4．64，11．26±4．14比12．82±3．85，9．72-3-2．25比13．30±2．83，9．12±2．72比13．10±2．72；IL-1β：83．49±12．79比100．09±17．53，85．10±11．75比102．64±19．64，75．97±11．29比99．24±14．62，81．96±14．81比99．59±12．05；TNF-α：8．95±1．13比9．94±1．29，8．79±1．80比9．56±0．89，8．27±1．83比9．51±1．32，7．23±1．39比9．23±1．05，P〈0．05或P〈0．01）；24h、72h时血必净低、中、高剂量组血清AST（U/L）和Cr（μmol／L）均显著降低（AST24h：24．00±1．27、23．80±1．11、22．90±1．65比39．50±1．73，72h：32．15±1．95、32．90±1．77、32．25±2．25比52．86±2．43；Cr24h：273．78±17．04、267．07±19．59、265．25±15．59比347．60±18．83，72h：437．38±18．48，343．77±16．79、351．48±20．22比437．50±19．86，均P〈0．01）。结论I／R损伤可导致明显Objective To investigate the changes in serum malondialdehyde （MDA）, interleukin-1β（IL-1β）, tumor necrosis faetor-α（TNF-α）, aspartate aminotransferase （AST） and creatinine （Cr） after the reproduction of ischemia/repeffusion （I/R） injury model, and the protective effects of liver and kidney with Xuebijing injection on acute I/R injury in rabbits. Methods Sixty rabbits were divided into sii groups with a random number： A, normal group; B, sham operated group; C, model group, and D, E, F groups （Xuebijing low, middle, high dosage treatment groups ）. I/R injury model was reproduced （after a 4-hour isehemia, the femoral vessels were reperfusion ）. Physiological saline （2 ml/kg） or 0.33, 0.66 and 1.32 g/kg Xuebijing injection were given at 0, 12, 36, 60 hours after operation via ear vein. MDA, IL-1β, TNF-α, AST and Cr were determined at 6, 12, 24 and 72 hours after reperfusion in each group. Results MDA, IL-1β, TNF-α at different time points, AST and Cr at 72 hours after reperfusion in C group were significantly higher than those in A group and B group. Compared with the C group, the above indexes were gradually decreased with does-dependence, the values of MDA （p, rnol/L）, IL-1β（ng/L） and TNF-α （μg/L） in serum of group F at 6, 12, 24 and 72 hours after reperfusion were significantly lower （MDA： 9.74 ±3.71 vs. 12.35 ± 4.64, 11.26± 4.14 vs. 12.82 ± 3.85, 9.72 ± 2.25 vs. 13.30 ± 2.83, 9.12± 2.72 vs. 13.10 ± 2.72; IL-I[3：83.49 ± 12.79 vs. 100.09 ± 17.53, 85.10 ± 11.75 vs. 102.64 ± 19.64, 75.97 ± 11.29 vs. 99.24 ± 14.62, 81.96 ± 14.81 vs. 99.59 ± 12.05 ; TNF-α： 8.95 ± 1.13 vs. 9.94 ± 1.29, 8.79 ± 1.80 vs. 9.56 ± 0.89, 8.27 ± 1.83 vs. 9.51 .± 1.32, 7.23 .± 1.39 vs. 9.23 ± 1.05, P〈0.05 or P〈0.01 ）. The values of AST（U/L） and Cr （p, mol/L） in serum of groups D, E and F at 24 hours and 72 hours after reperfusion were significantly lower （AST 24 hours： 24.00 ± 1.27, 23.80 ± 1.11, 22.90 ± 1.65 vs. 39.50 ± 1.73, 72

关 键 词：缺血/再灌注损伤 血必净注射液 丙二醛 白细胞介素-1Β 肿瘤坏死因子-Α 兔 

分 类 号：R363[医药卫生—病理学]