离子色谱法测定茶叶中的硝酸盐和亚硝酸盐  

Determination of Nitrate and Nitrite in Tea by Ion Chromatography

作　　者：蔡荟梅[1] 侯如燕[1] 高柱 彭传燚[1] 宛晓春[1] 

机构地区：[1]安徽农业大学教育部农业部茶叶生物化学与生物技术重点实验室,安徽合肥230036 [2]国家农副加工食品质量监督检验中心,安徽合肥230001

出　　处：《茶叶科学》2012年第2期95-99,共5页Journal of Tea Science

基　　金：农业部现代农业产业技术体系(农和教发[2008]10号);安徽省教育厅重点项目(KJ2011A105)

摘　　要：建立了离子色谱法测定茶叶中硝酸盐和亚硝酸盐的分析方法。样品经超声提取后,用PVPP吸附和固相萃取柱净化的方法进行前处理,经高容量阴离子交换柱分离,抑制型电导检测器检测。结果表明,样品中硝酸盐和亚硝酸盐的回收率范围分别为88.6%~107.5%和89.3%~101.2%。相对标准偏差（RSD）为1.67%和1.13%。离子色谱法测定硝酸根和亚硝酸根具有操作简便、测定快捷、准确性高的优点。A method for simultaneous determination of nitrite and nitrate in tea was established. Tea samples were pre-treated by ultrasonic extraction, PVPP and solid-phase extraction cartridge clean up, and the resulting supernatants were injected into a high-capacity anion exchange column with suppressed conductivity detection. The results showed that the recovery nitrate was 88.6%-107.5% and nitrite was 89.3%-101.2%. The relative standard deviations （RSD） were 1.67% and 1.13% respectively. It was concluded that the advantages of the method are simple operation, fast and high accuracy.

关 键 词：离子色谱法 茶叶 硝酸盐 亚硝酸盐 

分 类 号：TS272.7[农业科学—茶叶生产加工] O657.75[轻工技术与工程—农产品加工及贮藏工程]