Biotransformation of nitro-polycyclic aromatic compounds by vegetable and fruit cell extracts  

作　　者：Bo XIE Jun YANG Qing YANG 

机构地区：[1]School of Life Science and Biotechnology,Dalian University of Technology,Dalian 116024,China [2]National Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2012年第4期248-253,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Basic Research Program (973) of China (No.2010CB126102);the National Natural Science Foundation of China (No. 31070715);the National High-Tech R&D Program (863) of China (No.2011AA10A204);the National Key Technology R&D Program of China (No.2011BAE06B05);the Fundamental Research Funds for the Central Universities (No.DUT10LK33),China

摘　　要：Extracts from various vegetables and fruits were investigated for their abilities to reduce nitro-polycyclic aromatic hydrocarbons （NPAHs）. The extracts from grape and onion exhibited an interesting selectivity, yielding corresponding hydroxylamines or amines as major products under mild conditions of 30 ℃ and pH 7.0. Grape extracts reduced the 4-nitro-l,8-naphthalic anhydride with the highest conversion rate （〉99%） and the highest ratio of hydro- xylamine to amine （95：5）. In contrast, the onion extracts reduced 4-nitro-l,8-naphthalic anhydride with a conversion rate of 94% and a ratio of hydroxylamine to amine of 8：92. The thiol-reducing agent, 13-mercaptoethanol, and metal cations, Ca2＋ and Mg2＋, greatly increased the reductive efficiency. This work provides an alternative strategy for biotransformation of nitro-polycyclic compounds.Extracts from various vegetables and fruits were investigated for their abilities to reduce nitro-polycyclic aromatic hydrocarbons(NPAHs).The extracts from grape and onion exhibited an interesting selectivity,yielding corresponding hydroxylamines or amines as major products under mild conditions of 30 °C and pH 7.0.Grape extracts reduced the 4-nitro-1,8-naphthalic anhydride with the highest conversion rate(>99%) and the highest ratio of hydro-xylamine to amine(95:5).In contrast,the onion extracts reduced 4-nitro-1,8-naphthalic anhydride with a conversion rate of 94% and a ratio of hydroxylamine to amine of 8:92.The thiol-reducing agent,β-mercaptoethanol,and metal cations,Ca2+ and Mg2+,greatly increased the reductive efficiency.This work provides an alternative strategy for bio-transformation of nitro-polycyclic compounds.

关 键 词：BIOTRANSFORMATION Nitro-polycyclic aromatic compounds Plant cell extracts HYDROXYLAMINE AMINE 

分 类 号：TQ655[化学工程—精细化工] X132[环境科学与工程—环境科学]