新型微梁阵列生化传感器的研制  

A Novel Microcantilever Array Biochemical Sensor

作　　者：邬林[1] 周夏荣[1] 吴尚犬[1] 王萍[2] 张青川[1] 伍小平[1] 

机构地区：[1]中国科学技术大学,中科院材料力学行为和设计重点实验室,合肥230027 [2]中国科学院上海应用物理研究所物理生物学实验室,上海201800

出　　处：《分析化学》2012年第4期493-497,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.11127201)资助

摘　　要：为了消除单微梁生化传感系统中存在的温度漂移、溶液折射率变化等环境噪声影响,同时实现多种靶标分子的快速并行检测,设计制作了新型微梁阵列生化传感器。利用压电驱动激光束扫描微梁阵列,并通过光杠杆法实时读出微梁弯曲信号,即可得到在微梁表面发生的特异性生化反应的动力学曲线。对250!m间距的两定点的9h扫描实验数据验证了系统光路的稳定性;同时进行了温度激励测试,升温6℃后微梁阵列弯曲信号基本保持一致(误差6.5%),验证了系统检测的可靠性。最后,利用自制毛细管阵列套合修饰装置,成功将克伦特罗抗体修饰到微梁阵列一侧的金表面上,对待测液中10!g/L克伦特罗标样进行了准确检测,验证了此传感系统在生化检测中的实际可行性。To eliminate the impact of environmental noise such ,as temperature drift and change of liquid refractive index in the single microcantilever biochemical sensing system and achieve rapid parallel detection of a variety of target molecules, a novel microcantilever array biochemical sensor was designed and manufactured. When the mieroeantilever array was scanned by a piezoelectric-driven laser beam, the kinetic curves c f the specific biochemical reaction on the microcantilever array surface was obtained by real-time monitoring the deflections of the microcantilew＇.rs with the optical lever read-out technique. In the experiment, the system optical stability was verified by 9 h scanning of two fixed points with 250 μm pitch, and also the system detection reliability wa：s verified by the tempera- ture excitation test, in which the mieroeantilever array deflections were basically consistent with each other （error 6.5%） when heating up 6 ℃. Finally, 10 μg/L of c：lenbuterol antigen was detected by immobilizing clenbuterol antibody on the gold-coated side of the microcantilever array with the selfmade capillary array modification device, which verified the practical feasibility of the microcantilever array sensor.

关 键 词：生化传感器 无标记 微梁阵列 压电驱动 光杠杆法 毛细管阵列 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]