Midkine反义寡聚脱氧核苷酸对糖尿病肾病大鼠肾脏CTGF表达水平的影响  

Effect of midkine antisense oligonucleotides on the expression of CTGF in kidney of rats with diabetic nephropathy

作　　者：王俊伟[1] 张丽梅[1] 杨慧[2] 苑记清[1] 任晓军[1] 冯凭[1] 

机构地区：[1]天津医科大学总医院代谢病科,天津300052 [2]天津医科大学解剖学教研室,天津300070

出　　处：《天津医科大学学报》2012年第1期21-23,共3页Journal of Tianjin Medical University

基　　金：天津市卫生局科技基金资助项目(09kz113)

摘　　要：目的:研究中期因子(MK)反义寡聚脱氧核苷酸(AS-ODNs)对早期糖尿病肾病(DN)大鼠肾脏结缔组织生长因子(CTGF)表达水平的影响,揭示MK AS-ODNs在早期DN中的治疗前景。方法:将SD大鼠完全随机分为正常组、DN组和MK AS-ODNs组各20只,DN组和MK AS-ODNs组行单肾切+高糖高脂饲料+链脲佐菌素(STZ)建立糖尿病模型。成模第6周,MK AS-ODNs组尾静脉注射MK AS-ODNs,正常组和DN组予等量生理盐水,干预4 d后处死并留取肾脏组织,利用实时荧光定量PCR技术检测肾脏组织MK及CTGF的表达情况。结果:DN组大鼠肾脏MK和CTGF的表达水平较正常组明显增多且差异有统计学意义(P<0.05);MK AS-ODNs组大鼠肾脏MK和CTGF的表达水平较DN组明显减少且差异有统计学意义(P<0.05);MK与CTGF在大鼠肾脏组织的表达呈正相关(r=0.498,P<0.05)。结论:早期DN大鼠肾脏MK和CTGF的表达增多,MK AS-ODNs通过抑制MK的合成降低CTGF的表达水平,延缓DN进展。Objective： To investigate the effect of midkine（MK） antisense oligodeoxynucleortides（AS-ODNs） on the expression of connective tissue growth facter（CTGF） in kidney of rats with diabetic nephropathy（DN）.Methods： Sprague-Dawlry rats were randomly divided into normal control group（n=20）,DN group（n=20） and MK AS-ODNs group（n=20）.DN group and MK AS-ODNs group were treated with high-glucose and high-fat diet and intravenous injection STZ after unilateral nephritic excision.At the end of 6th week after diabetes mellitus model established,rats in MK AS-ODNs group were administrated with MK AS-ODNs,while normal control group and DN group were administrated with saline.After 4 days of intervention,the kidneys were rapidly dissected and stored for immunohistochemical and fluorescence quantitative PCR analyses.Results： The expression of MK and CTGF in DN group were increased significantly compared with that in normal group（P0.05）.The expression of MK and CTGF in kidney of MK AS-ODNs group were decreased compared with that in DN group（P0.05）.There were positive correlations between the expression of MK and CTGF in kidney of rat（r=0.498,P0.05）.Conclusion： The expression of MK and CTGF in rats with DN are increasing,MK AS-ODNs can inhibit the expression of MK and CTGF and then slow down the progress of DN.

关 键 词：糖尿病肾病 中期因子 结缔组织生长因子 反义寡聚脱氧核苷酸技术 大鼠 

分 类 号：R587.1[医药卫生—内分泌]