肝纤维化大鼠肝组织Raf/ERK信号通路的变化及肝复康的干预作用  

Inhibition of Gan-fu-kang on Raf/ERK signal transduction pathway in rats with hepatic fibrosis

作　　者：李骢[1] 雷鸿雁 姜妙娜[1] 张彩华[1] 贾玉杰[1] 

机构地区：[1]大连医科大学病理生理学教研室,辽宁省大连市116044 [2]辽宁省沈阳市金秋医院

出　　处：《实用肝脏病杂志》2012年第2期108-110,共3页Journal of Practical Hepatology

基　　金：辽宁省教育厅高等学校科研项目(编号:2009A190)

摘　　要：目的探讨中药肝复康通过Raf/ERK介导的信号转导通路治疗肝纤维化大鼠的作用机制。方法制备四氯化碳诱导的肝纤维化大鼠,应用肝复康干预;采用RT-PCR法测定肝组织及HSC-T6细胞Raf、ERK和Ⅲ型胶原mRNA水平的变化;采用免疫组织化学法检测肝组织ERK的表达。结果模型组肝组织Raf、ERK和Ⅲ型胶原mRNA水平较正常对照组明显上调(P<0.01),而肝复康治疗组则明显下调(P<0.01);模型组肝组织ERK蛋白的表达上调,而肝复康治疗组表达则下调。结论肝复康对肝纤维化有明确的治疗作用,其抗肝纤维化的作用机制可能与ERK介导的信号转导通路有关。Objective To investigate the mechanisms by which Gan-fu-kang inhibiting Raf/ERK signal transduction pathway in rats with hepatic fibrosis.Methods The rats with liver fibrosi were made by subcutaneously injecting with 10%CCl4 and treated with Gan-fu-kang.Expression of Raf,ERK and collagen Ⅲ mRNA were detected by RT-PCR.The ERK expression in liver tissues were detected by immunohistochemistry staining.Results The levels of Raf,ERK and collagen Ⅲ mRNA in model group increased than in control group（P0.01）;In the immunohistochemistry staining,the expression of ERK protein in model group was obviously elevated than in Gan-fu-kang treatment group.Conclusion Ganfukang has effective treatment on liver fibrosis and its treatment mechanisms probably involved in ERK-induced signal transduction pathway.

关 键 词：肝纤维化 肝星状细胞 肝复康 细胞外信号调节激酶 信号转导 

分 类 号：R575.2[医药卫生—消化系统]