伞形花内酯对植物病原真菌的抑制作用  

Antifungal activity of umbelliferone to plant pathogenic fungi

作　　者：白雪娜[1,2,3] 卜春亚[2] 谷继成[1,2] 梁为[4] 师光禄[2] 王有年[1,2] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]农业部都市农业(北方)重点开放实验室,北京102206 [3]广东省农业科学院科技情报研究所,广州510640 [4]北京林业大学林学院,北京100083

出　　处：《植物保护》2012年第2期42-45,共4页Plant Protection

基　　金：国家自然科学基金(30872029);北京市自然科学基金重点项目(6071001);北京市自然科学基金(6092007);北京市教委重点项目(KZ201010020016);北京市教委平台建设项目;北京市属高校人才强教深化计划(PHR20090516,PHR200906134)

摘　　要：[目的]研究伞形花内酯对桃褐腐病菌、棉花红腐病菌、草莓灰霉病菌、辣椒炭疽病菌的抑制活性,探究其对前两种病菌的抑菌机理。[方法]采用生长速率法测定伞形花内酯对4种植物病原真菌的抑制活性;采用二倍稀释法测定最小抑菌浓度和孢子萌发率;采用扫描电镜和透射电镜观察伞形花内酯对前两种病原菌超微结构的破坏。[结果]伞形花内酯对前两种植物病原菌具有较强的抑制作用,它们的MIC为250μg/mL和500μg/mL。后两种植物病原菌的MIC分别为1 000、2 000μg/mL。在伞形花内酯的作用下,菌丝外观出现变形、内容物外溢。菌丝内部出现空腔,细胞器降解,胞质凝集、出现流失,细胞结构遭到破坏,致使细胞死亡。[结论]伞形花内酯具有较强的抗菌活性,具有作为植物源杀菌剂的开发潜力。[Objective] To investigate the inhibitory activity of umbelliferone against Monilia fructigena,Fusarium moniliforme,Botrytis cinerea and Colletotrichum capsici,and study the anti-bacterial mechanism against the first two fungi.[Method] Growth rate method was used to test resistance to umbelliferone and doubling dilution method was used to determine the minimal inhibitory concentration（MIC） and spore germination.The ultrastructure of the first two fungi treated by umbelliferone was observed through scanning electron microscope（SEM） and transmission electron microscope（TEM）.[Result] Umbelliferone displayed strong antifungal activity to the first two plant pathogenic fungi and the MIC values were 250 and 500 μg/mL.The observations with SEM revealed that the hyphae were distorted,the mycelia were adhered together,cell surfaces were coarse and sunken,organelles were degraded,many blank areas appeared,cytoplasm agglutinated,and the cell structure was destroyed,leading to dead cells,based on TEM observations.[Conclusion] Pyrolin had a strong antifungal activity and could be exploited as a new resource.

关 键 词：伞形花内酯 植物病原菌 抑菌活性 超微结构 

分 类 号：S482.292[农业科学—农药学]