肺癌中LKB1蛋白表达的研究  

Expression of LKB1 protein in lung cancer

作　　者：朱延玲[1] 程元光[2] 黄大可[3] 

机构地区：[1]安徽医学高等专科学校,合肥230601 [2]合肥市第一人民医院,230061 [3]安徽医科大学基础医学院综合实验室,合肥230022

出　　处：《安徽医学》2012年第3期257-259,共3页Anhui Medical Journal

基　　金：安徽省高等学校省级自然科学研究项目(项目编号:KJ2008B222)

摘　　要：目的研究肿瘤抑制基因LKB1蛋白在肺癌中的表达。方法应用免疫组化方法检测肿瘤抑制基因LKB1蛋白在34例肺癌组织中的表达并结合肺癌的临床病理学特征进行分析。27例非肿瘤患者肺组织作对照。结果实验组34例LKB1蛋白阳性表达的平均光密度值为(0.273±0.056),对照组27例平均光密度值为(0.369±0.022),正常对照组表达强度显著高于实验组,两者比较差异有统计学意义(t=5.177,P=0.000);在肺癌中,LKB1蛋白的表达与肿瘤的恶性程度有显著的相关性(t=2.364,P=0.033),并且和淋巴结阳性与否关系密切(t=2.181,P=0.047)。结论 LKB1蛋白的表达下降可能在肺癌的发生、发展、转移中起重要作用,有可能成为肺癌诊断的参考指标和基因治疗的重要靶点。Objective To study the expression of LKB1 protein in lung cancer in order to investigate the role of LKB1 protein in the pathogenesis and progression in lung cancer.Methods Immunohistochemical staining was performed with LKBl antibody to the surgically resected tissue samples from 34 patients with lung cancer,and relation was analysed between the expression of LKB1 and clinical characteristics of lung cancer.Results In the experiment of attachment,the mean OD value of the experimental group（0.273±0.056） was significantly lower than that of the controls（0.369±0.022）.The expression level of LKB1 protein in lung cancer was significant lower than that in normal control groups（P0.05）.In lung cancer,the expression of LKBl protein was associated with the differentiated degree（t=2.364,P=0.033）,as well as extent of lymph node metastasis（t=2.181,P=0.047）.Conclusion Loss expression of LKBl may play an important role in the pathogenesis and development of lung cancer,and this may be a crucial reference marker for the prognosis of patients with lung cancer,and a target point for gene therapy.

关 键 词：肺癌 LKB1蛋白 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]