CD54和CD106在流感病毒FM1感染小鼠咽部黏膜上的表达  

Expression and significance of CD54 and CD106 in pharyngeal mucosa of mice infected with influenza virus FM1

作　　者：钟燕春[1] 杨进[1] 龚婕宁[1] 姜淼[1] 马可迅[1] 倪秋勤[1] 佟书娟[1] 

机构地区：[1]南京中医药大学,南京210046

出　　处：《中华中医药杂志》2012年第4期1214-1216,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：南京中医药大学科技创新风险基金项目(No.CX200902);江苏省2011年度普通高校研究生科研创新计划项目(No.CXZZ11_0757);江苏省高校优势学科(中西医结合学科)建设工程资助项目~~

摘　　要：目的:研究咽部黏膜细胞中细胞间黏附分子-1(CD54)、血管细胞黏附分子-1(CD106)在小鼠流感病毒H1N1型FM1感染中的表达及其意义。方法:18～20g清洁级昆明小鼠40只,随机分为实验组和对照组,采用酶联免疫吸附试验(ELISA)检测和采用免疫组化技术检测咽部黏膜上CD54、CD106的表达。结果:ELISA检测实验组小鼠黏膜匀浆中CD54含量,免疫组化检测实验组小鼠黏膜细胞中CD54、CD106含量与对照组比较明显升高(P<0.01)。结论:CD54、CD106在小鼠流感病毒FM1感染中发挥了重要作用,有助于实验判断病毒感染的程度,对于治疗和预防病毒感染提供重要信息。Objective： To detect the CD54 and CD106 on the surface of pharyngeal mucosa in mice infected with influenza virus FM1.Methods： 40 Kunming mice of clean grade,weighed 18~20g,were divided into experimental group and control group.The expressions of CD54 and CD106 on the surface of pharyngeal mucosa were detected with ELISA and immunohistochemistry.Results： CD54 in the mucosal homogenate of the mice with ELISA detection was significantly higher than that in the control group（P0.01）.CD54 and CD106 in the mice mucosal cell surface with immunohistochemical detection were significantly higher than the control group（P0.01）.The increased content of CD54 and CD106 were positively related with lung index.Conclusion： CD54 and CD106 play an important role in the FM1 infection and help to determine the extent of viral infection,and provide important information for the treatment and prevention of viral infection.

关 键 词：CD54 CD106 流感病毒 咽 黏膜 免疫组织化学 酶联免疫吸附试验 

分 类 号：R511.7[医药卫生—内科学]