瑞香狼毒根的超临界萃取物抑菌活性和成分分析  

Antifungal Activity of Extracts Obtained with Supercritical Carbon Dioxide Extraction from Roots of Stellera chamaejasme and Analysis of Their Constituents Using GC-MS

作　　者：白雪娜[1,2,3] 谷继成[1,2] 成军[1,4] 师光禄[1,4] 王有年[1,2] 

机构地区：[1]农业部都市农业(北方)重点开放实验室,北京102206 [2]北京农学院植物科学技术学院,北京102206 [3]广东省农业科学院科技情报研究所,广州510640 [4]北京农学院生物技术学院,北京102206

出　　处：《林业科学》2012年第2期169-174,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30872029);北京市自然科学基金重点项目(6071001);北京市自然科学基金项目(6092007);北京市教委重点项目(KZ201010020016);北京市教委科研基地-科技创新平台建设项目;北京市教委科技成果转化与产业化项目(PXM2011-014207-000026);北京市属高校人才强教深化计划资助项目(PHR20090516;PHR200906134);北京市科委重点项目(Z11100066111009)

摘　　要：瑞香狼毒（Stellera chamaejasme）为瑞香科（Thymelaeaceae）植物,广泛分布于我国西北、华北等地（刘欣等,2004）。据《神农百草经》记载,瑞香狼毒与大戟科（Euphorbiaceae）植物狼毒大戟（Euphorbia fischeriana）、月腺大戟（E.ebracteolata）同作狼毒入药,其性味苦平,全草有毒,有逐水祛痰、破积杀虫之功效（张慧文等,2006）。 瑞香狼毒也是一种极具开发潜力的植物源农药。In this work the supercritical fluid extraction with carbon dioxide was applied to extract roots of Stellera chamaejasme.The extracts were evaluated through the antifungal activity against Monilinia fructicola.Furthermore,the active compounds from the extracts were separated by macroporous resin and analyzed using gas chromatography-mass spectrometry（GC-MS）.The results showed that the extracts had strong antifungal activity against M.fructicola with inhibition ratio of 88.71% at 2 000 μg·mL-1,minimum inhibitory concentration（MIC） of 250 μg·mL-1,and minimum fungicidal concentration（MFC） of 2 000 μg·mL-1.After separated with macroporous resin chromatography,fourteen fractions were collected from the extracts.Fraction 4,7,and 12 in a concentration of 2 000 μg·mL-1 presented highly antifungal activity against M.fructicola,with inhibition ratio of 83.97%,80.23%,and 75.69%,respectively.The GC-MS analysis revealed that the main active compounds from fraction 4,7,and 12 included hexanedioic acid;bis（2-ethylhexyl） ester;β-sitosterol;7-methyl-Z-tetradecen-1-ol acetate9;9-hexadecenoic acid,hexadecyl ester,（Z）;1,2-benzenedicarboxylic acid,diisooctyl ester;（3β,24Z）,stigmasta-5,24（28）-dien-3-ol;stigmastan-3,5-diene,and squalene.

关 键 词：瑞香狼毒 超临界萃取 抑菌活性 桃褐腐病菌 

分 类 号：S767.1[农业科学—森林保护学]