贵州南、北盘江地区姜黄属植物中姜黄素类化合物含量对比研究  

Comparative Study on Curcumin of Curcuma Species from Nanpanjiang and Beipanjiang District,Guizhou Province

作　　者：叶世芸[1] 苏彦雷[1] 黄勇其[1] 徐文芬[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《安徽农业科学》2012年第12期7058-7060,共3页Journal of Anhui Agricultural Sciences

基　　金：贵州省科学技术基金资助项目(黔基合计字19993011号)

摘　　要：[目的]测定贵州南北盘江地区姜黄属植物中姜黄素类化合物的含量,为该地区药材品质评价提供科学参考依据。[方法]采用高效液相色谱法测定贵州南北盘江地区6种姜黄属植物(根及/或根茎)中姜黄素、去甲姜黄素、双去甲姜黄素的含量,色谱柱为InertsilODS-18色谱柱,流动相为无水甲醇-异丙醇-水-冰醋酸(20∶27∶48∶5,V/V/V/V),流速为0.5 ml/min,检测波长为420 nm。[结果]采自贵州安龙的姜黄其姜黄素类化合物含量最高,为5.94%;采自贞丰的郁金其姜黄素类化合物含量低。采自兴义不同基原的郁金中姜黄素类化合物的含量不同;姜黄中的含量最高(3.27%);毛郁金的次之(0.005 4%);莪术中的最低(0.001 5%)。[结论]该方法简便快捷,可为贵州南北盘江地区姜黄属植物药材的品质评价提供科学的参考依据。[Objective] To study the content of curcumin in Curcuma species sampled from Nanpanjiang and Beipanjiang District,Guizhou Province,so as to provide scientific basis for quality evaluation of related medicinal materials.[Method] The content of curcumin,demethoxycurcumin and bisdemethoxycurcumin in the root and/or rhizome of six Curcuma species was detected by HPLC.HPLC analysis was performed on Inertsil ODS-3 column with detection wavelength at 420 nm.The mobile phase consisted of anhydrous methanol,isopropyl alcohol,water and acetic acid（V/V/V/V,20∶ 27∶ 48∶ 5）,and the flow rate were 0.5 ml/min.[Result] The content of curcumin came from Anlong species was higher than others 5.94%,and the one came from Xingyi and Zhenfeng was lowest.Different original plants of RADIX CURCUMAE sampled from Xingyi varied greatly in the content of curcumin.Of these,the content of curcumin was the highest in RHIZOMA CURCUMAE LONGAE（3.27%）,followed by RADIX CURCUMAE（0.005 4%） and RHIZOMA CURCUMAE（0.001 5%）.[Conclusion] This method is simple and fast.The experimental result can provide scientific reference for quality evaluation of Curcuma medicinal materials from Nanpanjiang and Beipanjiang District,Guizhou Province.

关 键 词：贵州南北盘江地区 姜黄属 姜黄素类化合物 高效液相色谱法 

分 类 号：S567.9[农业科学—中草药栽培]