HPLC-ELSD法测定落叶松多糖中L-阿拉伯糖和D-半乳糖  

Determination of L-arabinose and D-galactose in polysaccharide of Larix gmelini by HPLC-ELSD

作　　者：王畅[1] 於洪建[2] 吴巍[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津尖峰天然产物研究开发有限公司,天津300457

出　　处：《现代药物与临床》2012年第2期117-119,共3页Drugs & Clinic

摘　　要：目的建立HPLC-ELSD法测定落叶松多糖中L-阿拉伯糖和D-半乳糖。方法色谱柱为Prevail Carbohydrate ES(250mm×4.6 mm,5μm),流动相为80%乙腈-0.5%乙酸铵水溶液(90∶10),体积流量1.0 mL/min,柱温室温,进样量20μL;检测器漂移管温度为100℃,载气体积流量为2.5 L/min。结果 L-阿拉伯糖、D-半乳糖分别在2.004 5～20.449 5μg(R2=0.9905)、2.041 0～20.410 2μg(R2=0.995 3)线性关系良好,平均加样回收率分别为99.68%(RSD为1.51%)、99.65%(RSD为1.63%)。结论本法重现性好,稳定可靠,可作为落叶松多糖的质量控制方法。Objective To establish a method for HPLC-ELSD determination of L-arabinose and D-galactose in polysaccharide of Larix gmelini.Methods The condition for HPLC-ELSD was Prevail Carbohydrate ES column（250 mm × 4.6 mm,5 μm） with 80% acetonitrile-0.5% ammonium acetate solution（90∶10） as the mobile phase.The flow rate was 1.0 mL/min.The column temperature was room temperature and the detector drift tube temperature was 100 ℃.The carrier gas flow velocity was 2.5 L/min,and the injection volume was 20 μL.Results The L-arabinose and D-galactose showed good linear relationship in the range of 2.004 495—20.449 5 μg（R2 = 0.990 5） and 2.041 0—20.410 2 μg（R2 = 0.995 3）,respectively;The average recoveries were 99.68%（RSD=1.51%） and 99.65%（RSD=1.63%）.Conclusion The method is reproducible,reliable,and can be used for the quality control for polysaccharide of L.gmelini.

关 键 词：落叶松多糖 L-阿拉伯糖 D.半乳糖 高效液相色谱一蒸发光散射检测 

分 类 号：R286.01[医药卫生—中药学]