RAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C.elegans  

作　　者：FENG Wan Juan LIANG Tao YU JunWei ZHOU Wei ZHANG YongDeng WU ZhengXing XU Tao 

机构地区：[1]College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China [2]National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Science China(Life Sciences)》2012年第3期228-235,共8页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China(Grant No. 2010CB833701);the National Natural Science Foundation of China(Grant Nos. 30870564 and 90913022);the CAS Project(Grant No.KSCX2-SW-224)

摘　　要：The molecular mechanisms by which dense core vesicles(DCVs) translocate,tether,dock and prime are poorly understood.In this study,Caenorhabditis elegans was used as a model organism to study the function of Rab proteins and their effectors in DCV exocytosis.RAB-27/AEX-6,but not RAB-3,was found to be required for peptide release from neurons.By analyzing the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy,we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking.The molecular mechanisms by which dense core vesicles （DCVs） translocate, tether, dock and prime are poorly understood. In this study, Caenorhabditis elegans was used as a model organism to study the function of Rab proteins and their effectors in DCV exocytosis..RAB-27/AEX-6, but not RAB-3, was found to be required for peptide release from neurons. By analyzing the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy, we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking.

关 键 词：EXOCYTOSIS dense core vesicles RAB-3 RAB-27 C. elegans total internal reflection fluorescence microscopy 

分 类 号：Q959.17[生物学—动物学] TM933.2[电气工程—电力电子与电力传动]