Acylation of Quercetin with a Novel Thermophilic Esterase as Biocatalyst  

作　　者：XIE Xiao-na ZHANG Chun-li XUN Er-na WANG Jia-xin ZHANG Hong WANG Lei WANG Zhi 

机构地区：[1]The First Hospital of Jilin University,Changchun 130021,P.R.China [2]Key Laboratory for Molecular Enzymology and Engineering,Ministry of Education [3]College of Physical Education,Jilin University,Changehun 130012,P.R.China

出　　处：《Chemical Research in Chinese Universities》2012年第2期225-229,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.31070708,21172093 and 20772046);the Natural Science Foundation of Jilin Province,China(No.201115038)

摘　　要：The regioselective acylation of quercetin catalyzed by a novel thermophilic esterase（APE1547） from the archaeon Aeropyrum pernix K1 was successfully conducted in organic solvents.The effects of acyl donor,substrate ratio,organic solvent,temperature,and water activity were investigated.Under the optimum conditions,a yield of 74% for its mono ester could be achieved in the reaction for about 6 h.With the reaction time extending to about 30 h,the final conversion of quercetin was about 100% and three products were synthesized.The regioselective acylation of quercetin catalyzed by a novel thermophilic esterase（APE1547） from the archaeon Aeropyrum pernix K1 was successfully conducted in organic solvents.The effects of acyl donor,substrate ratio,organic solvent,temperature,and water activity were investigated.Under the optimum conditions,a yield of 74% for its mono ester could be achieved in the reaction for about 6 h.With the reaction time extending to about 30 h,the final conversion of quercetin was about 100% and three products were synthesized.

关 键 词：QUERCETIN Vinyl acetate APE1547 ACYLATION 

分 类 号：TQ033[化学工程] Q946.833[生物学—植物学]