戊四氮点燃致发育期癫（疒间）大鼠海马神经元突触结构的改变  

Variation of hippocampal synaptic structure in pentylenetetrazol kindled epilepsy for developmental rats

作　　者：耿丽娟[1] 李慧[1] 王娟[1] 董洪洋[1] 王增芹[1] 袁宝强[1] 

机构地区：[1]徐州医学院附属医院儿科,江苏徐州221002

出　　处：《徐州医学院学报》2012年第2期84-87,共4页Acta Academiae Medicinae Xuzhou

摘　　要：目的 观察戊四氮（PTZ）点燃后发育期癫（疒间）大鼠海马神经元海马突触素（P38）和突触后致密物质（PSD95）及电镜超微结构的变化.方法 21日龄SD大鼠随机分为生理盐水组（NS）和实验组（PTZ）,用戊四氮建立点燃模型,免疫组化检测大鼠P38及 PSD95阳性细胞的数量及分布,电镜观察海马CA3区超微结构的变化.结果 PTZ组大鼠海马区P38及PSD95免疫反应产物表达量较NS组明显减少（P〈0.05）;电镜下NS组海马CA3区神经元形态及突触结构未见明显异常,PTZ组大鼠神经元细胞及突触结构均有改变.结论 戊四氮点燃所致癫（疒间）对发育期大鼠海马神经元的突触结构有明显损害.Objective To investigate the variation of hippocampal neurons P38 and PSD95 of pentylenetetrazol （PTZ） kindled epilepsy for developmental rats and change of electron microscopy ultrastructure. Methods 21 - day - old SD rats were randomly divided into normal saline group （NS） and experimental group （PTZ）. Pentylenetetrazol kin- dling model was established to detect immunohistochemically hippoeampal synaptophysin （ P38 ） and the number of posi- tive cells and distribution of postsynaptie density material （ PSD95 ）. Ultrastructural changes in hippocampal CA3 were observed by electron microscopy. Results P38 in hippoeampus of PTZ for 21 - day - old SD rats and expression in the immune reaction product of PSD95 was significantly reduced compared with that of NS group （ P 〈 0.05 ） ; Neuron mor- phology and synaptic structure in hippocampal CA3 of NS group had no obvious abnormalities under electron microscope ; Cells and synaptic structure of rats in PTZ group had been changed a little. Conclusion Epilepsy induced by pentyle- netetrazol kindling has significant structural damage to the synapses on hippocampal neurons.

关 键 词：癫(疒间) P38 PSD95 突触结构 

分 类 号：R742.1[医药卫生—神经病学与精神病学]