槲皮素对人宫颈癌HeLa细胞midkine和caspase-3表达的影响  

Effects of quercetin on the expression of midkine and caspase-3 on cervical cancer HeLa cells

作　　者：朱锦明[1] 张荣荣[1] 陆晓媛[2] 宋红娟[1] 薛华[1] 彭凤云[1] 拾莉[1] 

机构地区：[1]徐州市妇幼保健院妇产科,江苏徐州221009 [2]徐州医学院附属医院妇产科,江苏徐州221002

出　　处：《徐州医学院学报》2012年第2期116-119,共4页Acta Academiae Medicinae Xuzhou

基　　金：徐州市科技局科研课题（XZZD1032）

摘　　要：目的 通过观察槲皮素对宫颈癌 HeLa 细胞中midkine（MK）、caspase-3 基因和蛋白表达水平,探讨槲皮素体外诱导 HeLa 细胞凋亡的机制.方法 宫颈癌细胞株 HeLa 细胞经培养、传代后分为实验组（分别加入浓度为10、20、40、80及160 μmol/L的槲皮素）和空白对照组.采用逆转录-多聚酶链反应（RT-PCR）和蛋白质印迹（Western blot）方法检测细胞内的MK和caspase-3基因mRNA和蛋白表达水平.结果 随槲皮素浓度的增高HeLa细胞中MK mRNA和蛋白表达依次下降,其中80 μmol/L、160 μmol/L组下降较明显,而caspase-3的mRNA和蛋白表达随药物浓度增高表达增强（P〈0.01）.结论 槲皮素可能通过下调MK、上调caspase-3的表达水平而发挥抗宫颈癌作用.Objective To explore the influence of quercetin against cerical cancer possible mechanism of the apoptosis in vitro by observing the effects of quereetin （Que） on Midkine （MK） and caspase -3 genes and protein expression level of the human uterine cervix cancer HeLa cells. Methods HeLa cells cultured in vitro were divided into two groups： the experimental group and the control group. In experimental group, the cells were respectively treated with quercetin at different concentrations,10 μmol/L, 20 μmol/L, 40 μmol/L, 80 μmol/L and 160 μmol/L, while the cells in the control group weren＇t treated with quercetin. The expression of MK and easpase -3 mRNA and protein in HeLa cells was measured by reverse transcription（RT） - PCR and Western blot. Results Along with the increase of Que con- centration, MK mRNA and protein level down -regulated, in 80μmol/L and 160μmol/L group was significantly lower, but caspase - 3 mRNA and protein level obviously up - regulated （ P 〈 0.01 ）. Conclusion Que can induce the apoptosis of tumors through down - regulating MK expression and activating caspase - 3 expression.

关 键 词：槲皮素 MIDKINE CASPASE-3 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]