结直肠癌组织中miR-338-3p、SMO的表达变化及意义  

Expression and significance of miR-338-3p and SMO in colorectal carcinoma tissues

作　　者：苏桂源[1] 孙凯[1] 李国新[1] 余江[1] 

机构地区：[1]南方医科大学南方医院,广州510515

出　　处：《山东医药》2012年第14期1-3,共3页Shandong Medical Journal

基　　金：国家自然科学基金青年科学基金资助项目(81101896);广东省科技计划重点项目(2008A030201017);广东省医学科研基金资助项目(B2010190)

摘　　要：目的观察结直肠癌组织中微小RNA(miRNA)-338-3p(miR-338-3p)与Smoothened(SMO)的表达变化,并探讨其意义。方法 30例结直肠癌患者,手术时留取癌组织及癌旁组织,抽提其中的总RNA及蛋白质,采用实时荧光定量PCR检测标本中的miR-338-3p,用半定量RT-PCR检测SMO mRNA,Western blot检测SMO蛋白。结果结直肠癌组织中miR-338-3p的表达量(2-ΔΔCt)为0.153 7±0.126 4,SMO mRNA的相对表达量为0.371±0.116,SMO蛋白的灰度值为3.195±1.623,癌旁组织分别为0.901 5±0.426 3、0.366±0.117、0.733±0.305,两种组织中的miR-338-3p、SMO蛋白表达量相比,P均<0.01;miR-338-3p与SMO蛋白的表达呈负相关(r=-0.877,P<0.01)。结论结直肠癌组织中miR-338-3p表达明显下调,SMO蛋白表达明显上升;miR-338-3p可能通过转录后基因沉默机制使SMO蛋白表达下降,从而抑制结直肠癌的发生发展。Objective To investigate the expression and significance of microRNA（miRNA） -338-3p （miR-338-3p） and Smoothened （SMO） in colorectal carcinoma tissues. Methods The colorectal carcinoma and adjacent non-tumorous tissues were collected （from 30 patients ） respectively and the total RNA and protein were extracted routinely. The miR-338-3p was detected by Real-time quantitative PCR. SMO mRNA and corresponding protein were detected by semi- quantitative RT-PCR and Western blot. Results In colorectal carcinoma tissues, the miR-338-3p, SMO mRNA and SMO protein was 0. 153 7 ± 0. 126 4, 0.371 ± 0.116, 3. 195 ±1. 623, respectively, whereas in the adjacent non-tumorous tis- sues, the miR-338-3p, SMO mRNA and SMO protein was 0.901 5 ±0.426 3, 0. 366±0. 117, 0.733 ±0. 305, respec- tively. The differeace between the expression of miR-338-3p and SMO protein in these two tissues was statistically signifi- cant （ all P 〈 0.01 ）. There was a remarkable inverse correlation between miR-338-3p and SMO protein levels was observed （ r = -0.877 ,P 〈0.01 ）. Conclusion The expression of miR-338-3p was significantly down-regulated in colorectal carci- noma tissues, whereas the SMO protein was markedly increased, miR-338-3p may inhibit SMO expression by post-tran- scriptional gene silencing to restrain eolorectal carcinoma occurrence and progress.

关 键 词：结肠肿瘤 直肠肿瘤 微小RNA Smoothened基因 

分 类 号：R735.3[医药卫生—肿瘤]