Cellular exposure to muscle relaxants and propofol could lead to genomic instabilityin vitro  

作　　者：Allen Edward Coleman Nicole McNeil Alexander Leonidovich Kovalchuck Dara Wangsa Thomas Ried Hong Wang 

机构地区：[1]Department of Anesthesiology,Wayne State University,Detroit Medical Center,Detroit,Michigan 48201,USA [2]Laboratory of Cancer Genomics,National Cancer Institute,National Institutes of Health,Bethesda,Maryland 20892,USA [3]Laboratory of Immunogenetics,National Institute of Allergy and Infectious Disease,National Institutes of Health,Rockville,Maryland 20852,USA

出　　处：《The Journal of Biomedical Research》2012年第2期117-124,共8页生物医学研究杂志（英文版）

基　　金：supported in part by the Intramural Research Program of the NIH,the National Cancer Institute and the National Institute of Allergy and Infectious Diseases

摘　　要：Anesthesia is widely used in several medical settings and accepted as safe. However, there is some evidence that anesthetic agents can induce genomic changes leading to neural degeneration or apoptosis. Although chromosomal changes have not been observed in vivo, this is most likely due to DNA repair mechanisms, apoptosis, or cellular senescence. Potential chromosomal alterations after exposure to common anesthetic agents may be relevant in patients with genomic instability syndromes or with aggressive treatment of malignancies. In this study, the P388 murine B cells were cultured in vitro, and spectral karyotyping （SKY） was utilized to uncover genomewide changes. Clinically relevant doses of cisatracurium and propofol increased structural and numerical chromosomal instability. These results may be relevant in patients with underlying chromosomal instability syndromes or concurrently being exposed to chemotherapeutic agents. Future studies may include utilization of stimulated peripheral blood lymphocytes to further confirm the significance of these results.Anesthesia is widely used in several medical settings and accepted as safe. However, there is some evidence that anesthetic agents can induce genomic changes leading to neural degeneration or apoptosis. Although chromosomal changes have not been observed in vivo, this is most likely due to DNA repair mechanisms, apoptosis, or cellular senescence. Potential chromosomal alterations after exposure to common anesthetic agents may be relevant in patients with genomic instability syndromes or with aggressive treatment of malignancies. In this study, the P388 murine B cells were cultured in vitro, and spectral karyotyping （SKY） was utilized to uncover genomewide changes. Clinically relevant doses of cisatracurium and propofol increased structural and numerical chromosomal instability. These results may be relevant in patients with underlying chromosomal instability syndromes or concurrently being exposed to chemotherapeutic agents. Future studies may include utilization of stimulated peripheral blood lymphocytes to further confirm the significance of these results.

关 键 词：spectral karyotyping （SKY） genomic instability epigenetic P388 PROPOFOL CISATRACURIUM VECURONIUM PANCURONIUM ANEUPLOIDY 

分 类 号：R614[医药卫生—麻醉学]