TNF-α、IL-1β 、LPS对人多核白细胞、脐静脉内皮细胞血小板内皮细胞粘附分子-1表达的影响  

TNF-α. IL-1β. LPS Affects of Expressics of Platelet Endothelial Cell Adhesion Molecule-1 in Human Polymorphonuclear and Umbilical Vein Endothelial Cells

作　　者：王建春 毛宝龄[1] 钱桂生[1] 

机构地区：[1]中国人民解放军第三军医大学新桥医院呼吸内科研究所,邮政编码重庆400037

出　　处：《微循环学杂志》2000年第1期6-8,共3页Chinese Journal of Microcirculation

摘　　要：目的：初探炎性刺激对人多核白细胞（ＰＭＮ）和脐静脉内皮细胞（ＨＵＶＥＣ）的血小板内皮细胞粘附分子－１（ＰＥＣＡＭ－１）表达的影响和ＰＥＣＡＭ－１的可能作用。方法：使用流式细胞仪和免疫组化法，观察脂多糖（ＬＰＳ）、白介素（ＩＬ）－１β和肿瘤坏死因子（ＴＮＦ）－ａ对ＰＭＮ和ＨＵＶＥＣ ＰＥＣＡＭ－１表达的变化。结果：ＬＰＳ、１Ｌ－１β和ＴＮＦ－ａ可引起ＰＭＮ表达ＰＥＣＡＭ－１蛋白减少，而对ＨＵＶＥＣ　ＰＥＣＡＭ－１的表达无显著影响，但组化显著在ＨＵ－ＶＥＣ连接部的ＰＥＣＡＭ－１染色变淡。结论：上述炎性刺激物不仅可引起ＰＭＮ表达ＰＥＣＡＭ－１减少、激活ＰＭＮ，而且可改变内皮细胞ＰＥＣＡＭ－１的分布，因此ＰＥＣＡＭ－１在炎症中可能起重要作用。Objective: Preliminarily effect of inflammation stimuli on platelet endothelial cell adhesion molecule-1 (PECAM-l) in polymorphonuclear(PMN) and human umbilical vein endothelial cells(HUVEC) and potential roles of PECAM-1 were explored. Method: Changes of PECAM-1 expression in PMN and HUVEC stimulated by lipopolyaccharide (LPS). interleukin-1β (IL-1β) and tumor necrosis factor-α(TNF-α) by flow cytometry and immunohistochemistry were observed. Results: LPS. IL-1β and TNF-α induced PECAM-l protein expression reduction in PMN, but did not affect significantly PECAM-1 expression in HUVEC. Immunohistochemistry re vealed reduced PECAM-1 staining in HUVEC junctions stimulated by inflammation stimuli. Conclusion: Inflammation stimuli above can cause not only reduction of PECAM-1 expression for activating PMN but also PECAM-l redistribution on HUVEC, these imply PECAM-1 maybe play an important role during inflammation.

关 键 词：炎症 TNF-α IL-1Β LPS PECAM-1 多核白细胞 

分 类 号：R364.5[医药卫生—病理学]