Study on the Metabolic Characteristics of Aconite Alkaloids in the Extract of Radix aconiti under Intestinal Bacteria of Rat by UPLC/MSn Technique  

作　　者：辛杨 皮子凤 宋凤瑞 刘志强 刘淑莹 

机构地区：[1]Changchun Center of Mass Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,5625 Renmin Street,Changchun,Jilin 130022,China [2]Graduate School of the Chinese Academy of Sciences,Beijing 100039,China

出　　处：《Chinese Journal of Chemistry》2012年第3期656-664,共9页中国化学（英文版）

摘　　要：The extract of Radix aconiti was incubated with rat intestinal bacteria in vitro. Further, aconitine and hypaconitine standard were incubated at the same condition as the extract of Radix aconiti, respectively. Ultra Performance Liquid Chromatography/Multi-Mass Spectrometry （UPLC/MSn） was used for detecting and identifying all the aco- nite alkaloids. Results showed that there were four metabolites which were identified as 8-butyryl-14-benzoylmesa- conine （m/z 660）, 8-propionyl-14-benzoylaconine （m/z 660）, 8-butyryl-14-benzoylaconine （m/z 674） and 8-valeryl-14-benzoylmesaconine （m/z 674） in the metabolized sample of the extract of Radix aconiti. The relative area ratio of them presented increasing trend during 6 d. On the basis of all results, we could concluded that substi- tution at N atom mainly influenced the metabolizing rate of diester-diterpenoid alkaloids （DDAs）, C-8 substitute was active metabolized site, intestinal bacterial metabolites of the aconite alkaloids in the extract of Radix aconiti were mainly the substitute with propionyl group, butyl group or valeryl group at C-8. This paper illustrated holistic metabolizing profile of the extract of Radix aconiti in vitro and possible metabolizing reaction type of main DDAs, which could provide reference for finding out potential bioactive components in the extract and the prescription of Chinese Medicine.The extract of Radix aconiti was incubated with rat intestinal bacteria in vitro. Further, aconitine and hypaconitine standard were incubated at the same condition as the extract of Radix aconiti, respectively. Ultra Performance Liquid Chromatography/Multi-Mass Spectrometry （UPLC/MSn） was used for detecting and identifying all the aco- nite alkaloids. Results showed that there were four metabolites which were identified as 8-butyryl-14-benzoylmesa- conine （m/z 660）, 8-propionyl-14-benzoylaconine （m/z 660）, 8-butyryl-14-benzoylaconine （m/z 674） and 8-valeryl-14-benzoylmesaconine （m/z 674） in the metabolized sample of the extract of Radix aconiti. The relative area ratio of them presented increasing trend during 6 d. On the basis of all results, we could concluded that substi- tution at N atom mainly influenced the metabolizing rate of diester-diterpenoid alkaloids （DDAs）, C-8 substitute was active metabolized site, intestinal bacterial metabolites of the aconite alkaloids in the extract of Radix aconiti were mainly the substitute with propionyl group, butyl group or valeryl group at C-8. This paper illustrated holistic metabolizing profile of the extract of Radix aconiti in vitro and possible metabolizing reaction type of main DDAs, which could provide reference for finding out potential bioactive components in the extract and the prescription of Chinese Medicine.

关 键 词：Radix aconiti ALKALOIDS intestinal bacteria liquid chromatography-mass spectrometry 

分 类 号：S963.161[农业科学—水产养殖] Q939.121[农业科学—水产科学]