下调SnoN基因表达对HepG2细胞增殖及凋亡的影响  

Effect of down-regulating SnoN gene expression on proliferation and apoptosis of HepG2 cells

作　　者：王坤红[1] 王文奇[2] 相玉芬[1] 王义国[2] 刘长虹[2] 李双玲[2] 曹莉莉[3] 

机构地区：[1]山东大学医学院,济南250012 [2]山东大学附属千佛山医院消化内科,济南250014 [3]山东大学附属千佛山医院中心实验室,济南250014

出　　处：《山东大学学报（医学版）》2012年第4期33-37,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技发展计划基金资助项目(2008GG10002047)

摘　　要：目的通过小干扰RNA(siRNA)下调人肝癌HepG2细胞中SnoN基因的表达,探讨SnoN对HepG2细胞增殖、凋亡等生物学功能的影响及其意义。方法设计并化学合成3条靶向SnoN的siRNA序列,采用阳离子脂质体瞬时转染的方法将干扰序列转染至HepG2细胞内,采用RT-PCR和Western blot方法,检测转染后的抑制效果并筛选出一条抑制效果最好的干扰序列,然后采用CCK-8法和流式细胞术检测SnoN基因表达下调后HepG2细胞增殖、凋亡的变化。结果 3条siRNA均对SnoN的表达有抑制作用,以siRNA-C抑制效果最好。下调SnoN基因表达后,HepG2细胞的增殖受到抑制(P<0.05),细胞凋亡增加(P<0.05)。结论靶向SnoN的siRNA可有效抑制SnoN基因的表达,进而使HepG2细胞的生长受到抑制、细胞凋亡增加,表明SnoN基因可能与肝癌细胞的增殖与凋亡有关。Objective To explore the biological influence of small interfering RNA（siRNA） down-regulating SnoN gene expression on biological function of human HepG2 cells,such as proliferation and apoptosis.Methods Three siRNAs targeting SnoN were designed and synthesized.The siRNAs were transiently transfected into HepG2 cells by cationic liposomes,and then the inhibition effect was detected by reverse transcription polymerase chain reaction（RT-PCR） and Western blot.The SnoN-siRNA with the most powerful inhibition was selected.The proliferation of HepG2 cells was examined by CCK-8,and the apoptosis was examined by flow cytometry.Results All three siRNAs efficiently inhibited expression of the SnoN gene,and the siRNA-C had the highest inhibition ratio.The proliferation of HepG2 cells was significantly inhibited（P0.05） and the apoptosis increased（P0.05） after SnoN was down-regulated.Conclusion SnoN specific siRNA can effectively inhibit expression of SnoN in human HepG2 cells,and induce growth inhibition and apoptosis.

关 键 词：小干扰RNA 基因 SnoN 肝肿瘤 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]