淋巴滞留性脑病对大鼠脑组织超微结构及心率变异性的影响  

Influences of lymphostatic encephalopathy on the ultrastructure and heart rate variability in rats

作　　者：郑冬雁[1] 潘燕[1] 刘尚明[2] 王立言[2] 孟延[1] 徐红岩[3] 李红宇[4] 魏蜀一[1] 

机构地区：[1]山东大学医学院生物医学工程研究所,济南250012 [2]山东大学医学院组织学与胚胎学教研室,济南250012 [3]山东大学医学院机能学实验教学中心,济南250012 [4]山东大学附属济南市中心医院医保办公室,济南250013

出　　处：《山东大学学报（医学版）》2012年第4期66-70,75,共6页Journal of Shandong University:Health Sciences

摘　　要：目的研究淋巴滞留性脑病对自主神经系统的影响。方法采用阻断颈部淋巴引流的方法将Wistar大鼠80只制作成淋巴滞留性脑病(LE)模型,并随机分为假手术组(SHAM组)和淋巴滞留性脑病组(LE组)各40只,每组又分为术后1、7、14、21 d 4个子组。采集肢体Ⅱ导联心电信号,进行心率变异性(HRV)分析,观察延髓头端腹外侧(RVLM)区脑组织形态学变化,用Western blot方法检测RVLM区eNOS蛋白的表达。结果与SHAM组相比,LE组心率变异性增大、心率减慢(P<0.05),迷走神经调节增强,RVLM区出现神经细胞变性坏死,毛细血管通透性增加,脑组织水肿,超微结构可见神经细胞凋亡、髓鞘破裂,eNOS蛋白表达下调。结论 LE造成大鼠脑组织结构与自主神经调节功能损伤,损伤的机制可能部分与下调eNOS蛋白表达有关。Objective To investigate the influences of lymphostatic encephalopathy（LE） on the autonomic nervous system.Methods The model of LE was established by occluding lymphatic vessels and removing both shallow and deep cervical lymph nodes in Wistar rats.The rats were randomly divided into the sham-operation（SHAM） group and the LE group,40 rats in each group.The electrocardiogram（ECG）（lead II） of rats was synchronously recorded by the PowerLab system on postoperative days 1,7,14 and 21.Heart rate variability（HRV） was analyzed with ECG signals.Brain morphology of the rostral ventrolateral medullas（RVLM） was observed by a light microscope and an electron microscope.eNOS protein expression in RVLM was determined by Western blot.Results Compared with the SHAM group,HRV was significantly increased,while HR was reduced（P0.05）.The vagus nerve modulation was enhanced,necrosis of neurons appeared in RVLM,permeability of capillaries was increased,and brain tissue edema occurred.Ultrastructural observation showed neuronal apoptosis and myelin sheath disintegration.Protein expression of eNOS was significantly decreased.Conclusion LE induces injury to the cerebral tissue and autonomic nervous regulation,and the mechanism may be related to down-regulation of eNOS expression.

关 键 词：淋巴滞留性脑病 心率 一氧化氮合酶 延髓头端腹外侧区 

分 类 号：R364.18[医药卫生—病理学]