帕罗西汀对慢性不可预见应激大鼠惊跳反射和弱刺激抑制影响的初步研究  

Effects of paroxetine on acoustic startle reflex in chronic unpredicted stress rats

作　　者：白渊翰[1] 薛昀赟[1] 彭正午[1] 王化宁[1] 陈云春[1] 谭庆荣[1] 

机构地区：[1]第四军医大学西京医院心身科,西安710032

出　　处：《中华精神科杂志》2012年第2期103-106,共4页Chinese Journal of Psychiatry

基　　金：国家科学自然基金（30870886,30700259）

摘　　要：目的探讨慢性不可预见应激大鼠的听觉惊跳反射和弱刺激抑制变化情况以及帕罗西汀对其的影响。方法将24只大鼠按随机数字表法分为阴性对照组（8只）、慢性不可预见应激组（8只）和帕罗西汀组（8只）。阴性对照组：静养21d后给予蒸馏水灌胃；慢性不可预见应激组：先给予慢性不可预见应激21d后再给予蒸馏水灌胃21d；帕罗西汀组：先给予慢性不可预见应激21d后再给予帕罗西汀灌胃21d。3组大鼠均接受体质量测量、自发活动、糖水偏好、惊跳反射和弱刺激抑制测试。结果（1）慢性不可预见应激组大鼠体质量[（380．50±22．23）g]、10min旷场自发活动[（5765．57±2942．28）mm]、糖水摄入量[（19．09±7．16）mL／kg]均低于阴性对照组[（426．38±33．73）g、（12272．15±2343．02）mm、（42．58±11．68）ml／kg，P〈0．01]．帕罗西汀组体质量[（353．62±29．37）g]低于阴性对照组（P〈0．01）。（2）惊跳反射实验结果3组大鼠之间差异无统计学意义（P〉0．05）。（3）慢性不可预见应激组大鼠弱刺激抑制[（30．50±14．84）％]小于阴性对照组和帕罗西汀组[（57．80±13．32）％、（42．32±15．82）％]，帕罗西汀组弱刺激抑制小于阴性对照组，差异均有统计学意义（P〈0．01）；82dB时的弱刺激抑制高于70、64、58dB时的弱刺激抑制（P〈0．01）；76dB时的弱刺激抑制高于64、58dB时的弱刺激抑制（P〈0．01）；70dB时的弱刺激抑制高于58（1B时的弱刺激抑制（P〈0．05）。结论随着弱刺激强度增加，弱刺激抑制逐渐增加；慢性不可预见应激大鼠存在弱刺激抑制的缺失，帕罗西汀能够缓解这一状况。Objective To explore the deficits of prepulse inhibition （PPI） of acoustic startle reflex （ASR） in chronic unpredicted stress （CUS） rats, and the effects of paroxetine on ASR and PPI. Methods Twenty-four Sprague Dawley rats were randomly divided into control group, CUS group and CUS ± paroxetine group. The rats in control group were left undisturbed in their home cage for 21 days and then administration of distilled water （5 ml/kg） for consecutive 21 days; the rats in CUS group exposed to 21 consecutive days of CUS and then treated with distilled water （5 ml/kg） for consecutive 21 days; the rats in CUS ± paroxetine group exposed to 21 consecutive days of CUS and then treated with paroxetine （10 mg/kg） mixed with distilled water （5 ml/kg） for consecutive 21 days. Behavioral changes in these rats were analyzed in weight change, open field test, sucrose preference, ASR and PPI paradigm. Results （ 1 ） Compared with control group, weight [ （380. 50 ± 22.23 ） g ], 10-min total traveling distance [（5765.57 ± 2942. 28 ） mm ], sucrose preference [ （ 19. 09 ± 7.16） ml/kg] were lower （ P 〈 0. 01 ） in the CUS group. Weight of CUS ± paroxetine group [ （353.62 ± 29.37） g] was lower than control group （ P 〈 0. 01 ）. （2） There were no differences of ASR among control group, CUS group, and CUS ± paroxetine group （ P 〉 0.05 ） . （3） Compared with control group and CUS ± paroxetine group, PPI in CUS group [ （30. 50 ± 14.84）% ] was reduced （P 〈 0. 01 ）, PPI in CUS ± paroxetine group was lower than control group （P 〈 0. 01 ）. PPI of 82 dB [ （56. 00 ± 17.10）% ] was higher than that of 70, 64, 58 dB （P 〈0. 01） ; PPI of 76 dB [ （49. 76 ± 18.03 ）% ] was higher than that of 64, 58 dB （P 〈 0. 01 ） ; PPI of 70 dB [ （43.17 ± 15.22） % ] was higher than that of 58 dB （ P 〈 0. 05 ） . Conclusion The results suggest there be impaired PPI in chronic unpredieted stress rats, while paroxetine could alleviate the defici

关 键 词：帕罗西汀 应激 惊吓反应 弱刺激抑制 感觉运动门控通道 

分 类 号：R74[医药卫生—神经病学与精神病学]