荧光探测法探测兔甲状腺前哨淋巴结  

Detection of sentinel lymph node of rabbit thyroid by fluorescence imaging

作　　者：柳桢[1] 王家东[1] 周争[1] 

机构地区：[1]上海交通大学医学院附属仁济医院耳鼻咽喉头颈外科上海交通大学耳鼻咽喉科研究所,200001

出　　处：《中华耳鼻咽喉头颈外科杂志》2012年第4期294-297,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

摘　　要：目的通过将荧光示踪剂吲哚花青绿（indocyanine green，ICG）及其与小牛血清白蛋白（calf serum albumin，CSA）的复合体ICG：CSA注射入兔甲状腺中，观察其探测兔甲状腺前哨淋巴结的效果，为使用荧光探测法探测甲状腺癌患者的前哨淋巴结提供动物实验依据。方法将35nmol／L的ICG1ml和35nmol／L的ICG：CSA1ml进行荧光成像，记录两者的荧光总量。20只实验兔单纯随机抽样方法分为两组，每组各10只实验兔，分别注射100nmol／LICG和35nmol／LICG：CSA0．02ml于兔单侧甲状腺组织内。再对该20只实验兔同侧甲状腺内注射美蓝0．02ml。观察注射ICG、ICG：CSA和美蓝后前哨淋巴结的检出情况。结果同等浓度和剂量的ICG：CSA溶液的荧光总量为ICG溶液的3倍。注射ICG和ICG：CSA的20只实验兔均有前哨淋巴结被检出，注射美蓝后有16只实验兔前哨淋巴结被检出。荧光探测法和美蓝探测法的准确率分别为95．8％和79．2％。结论ICG和ICG：CSA作为荧光示踪剂探测兔甲状腺的前哨淋巴结具有满意的检出率。荧光探测法探测甲状腺前哨淋巴结具备在未来应用于临床的可能。Objective To detect the sentinel lymph nodes （SLN） of rabbit thyroid by fluorescence imaging and to provide experimental evidence for its clinical application. Methods Equal concentration and dose （35 nmol/L, 1 ml） of ICG （indocyanine green） or ICG： CSA （calf serum albumin） complex was injected into rabbit thyroid to measure the their fluorescence amount. Twenty rabbits were randomly divided into ICG group and ICG： CSA group, with 10 rabbits each group, rabbit thyroid was separately injected with 0. 02 ml of 100 nmoL/L ICG and 0. 02 ml of methylene, or 0. 02 ml of 35 nmol/L ICG： CSA and 0. 02 ml of methylene, and then ICG fluorescence and methylene staining in SLN were detected respectively. Results Fluorescence amount of ICG： CAS was three times that of ICG with the same concentration and dose. SLNs were showed in all rabbits injected with ICG or ICG： CSA, but found only in 16 of 20 rabbits injected with methylene blue. The accuracy of fluorescence imaging and blue dye imaging were respectively 95.8% and 79.2%. Conclusion Fluorescence imaging, using ICG or ICG： CSA as a tracer, could be an alternative method for the detection of SLN of thyroid in future clinical practice.

关 键 词：甲状腺 淋巴结 荧光 吲哚花青绿 血清白蛋白 牛 前哨淋巴结活组织检查 

分 类 号：R737.9[医药卫生—肿瘤]