生鲜牛乳体系中黄体酮的检测方法  

Study on Progesterone Detection Methods in Milk

作　　者：赵晓宇[1] 张英华[1] 生庆海[2] 张志国[2] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]内蒙古蒙牛乳业(集团)股份有限公司研发中心,内蒙古呼和浩特011500

出　　处：《食品研究与开发》2012年第3期138-140,共3页Food Research and Development

摘　　要：采用超高压液相色谱—串联四级杆质谱仪（配有电喷雾离子源）对液态奶中的黄体酮进行检测,黄体酮为脂溶性物质,提取时加入少量氨水,使黄体酮从脂肪中释放的更完全,提取率更高。定量方法采用外标法定量,回收率最低为94.75%,最高为99%,RSD在14.82%~20.74%之间,使得检测结果更加准确,快捷。In this paper,the progesterone in milk was detected with UPLC-MS /MS（equipped with electrospray ionization source）.Progesterone is fat soluble material,extraction by adding a small amount of ammonia,the progesterone of the fat release more complete and the progesterone extraction rate was high.Using the external standard method for quantitative,the recoveries from 94.75 % to 99 %,RSD was 14.82 %-20.74 %.the detection result was more accurate and fast.

关 键 词：激素 色谱 研究 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]