蛇毒精氨酸酯酶Agkihpin上调人肝癌细胞株SMMC-7721中表皮钙黏素的表达研究  

Research on Agkihpin Up-Regulating the Expression of E-Cadherin in Hepatocellular Carcinoma SMMC-7721 Cell Strain

作　　者：黄程新[1] 许淑茹[2] 胡启平[1] 马军[1] 方玲[1] 袁志刚[1] 李晓龙[1] 

机构地区：[1]广西医科大学细胞生物学与遗传学教研室,广西南宁市530021 [2]漳州卫生职业学院

出　　处：《中国全科医学》2012年第9期1002-1005,共4页Chinese General Practice

基　　金：广西青年基金(桂科青0728059)

摘　　要：目的探讨蛇毒精氨酸酯酶Agkihpin对人肝癌SMMC-7721细胞株中表皮钙黏素(E-CD)表达的影响及Agkihpin抑制人肝癌SMMC-7721细胞的机制。方法采用不同浓度的Agkihpin处理肝癌细胞株72 h后,应用免疫细胞化学、Western Blotting和逆转录PCR(RT-PCR)法检测E-CD在SMMC-7721细胞中的表达。结果不同浓度Agkihpin作用SMMC-7721细胞72 h后E-CD表达均上升,差异有统计学意义(P<0.05),并呈现出一定的剂量依赖效应。Agkihpin可显著上调SMMC-7721细胞中E-CD表达。结论在人低分化肝癌SMMC-7721细胞株中,Agkihpin能促进E-CD的表达,因而可能抑制肝癌细胞的迁移和降低肝癌组织的恶性程度。Objective To explore the effects of Agkihpin on the expressions of E - Cadherin in hepatocellular carcino- ma SMMC -7721 cell strain and to describe the mechanism of Agkihpin inhibiting SMMC -7721. Methods The hepatocellular carcinoma cell strain was treated with different concentrations of Agkihpin for 72 h. The expressions of E - Cadherin in SMMC - 7751 cell strain were assayed by immunocytochemistry, Western Blotting and RT - PCR. Results E - Cadherin expressions in SMMC -7721 cells were all significantly increased after 72 h treatment by different concentrations of Agkihpin （P 〈0.05）, and dose dependent effect was shown in the process. Agkihpin could significantly increase the expressions of E- CD in SMMC -7721 cells. Conclusion Agkihpin can promote the expressions of E - Cadherin in SMMC -7721 cells, which might be one of the rea- sons for Agkihpin inhibiting the cell migration of hepatocellular carcinoma cell and decreasing the grade malignancy of hepatocellu- lar carcinoma.

关 键 词：表皮钙黏素 精氨酸酯酶 肝肿瘤 蛇毒液类 

分 类 号：R735.7[医药卫生—肿瘤]